{"id":28247,"date":"2026-05-26T17:49:17","date_gmt":"2026-05-26T08:49:17","guid":{"rendered":"https:\/\/nbsigh2.com\/?p=28247"},"modified":"2026-05-26T18:44:45","modified_gmt":"2026-05-26T09:44:45","slug":"stap%e7%b4%b0%e8%83%9e%ef%bc%94%e3%81%a4%e3%81%ae%e3%83%97%e3%83%ad%e3%83%88%e3%82%b3%e3%83%ab","status":"publish","type":"post","link":"https:\/\/nbsigh2.com\/?p=28247","title":{"rendered":"STAP\u7d30\u80de\uff14\u3064\u306e\u30d7\u30ed\u30c8\u30b3\u30eb"},"content":{"rendered":"

\u4ee5\u524d\u304b\u3089\u601d\u3063\u3066\u3044\u305f\u306e\u3067\u3059\u304c\u3001\u76db\u3093\u306b\u5b66\u3068\u307f\u5b50\u306f\u9178\u6d74\u306e\u65b9\u6cd5\u304c\u3001\u4ed6\u306e\u518d\u73fe\u5b9f\u9a13\u3092\u884c\u3063\u305f\u7814\u7a76\u8005\u306f\u518d\u73fe\u3057\u3066\u3044\u306a\u3044\u3068\u3044\u3046\u308f\u3051\u3067\u3059\u3002<\/p>\n

\u300c\u5206\u5316\u7d30\u80de\u3092\u57f9\u990a\u6db2\u306b\u3064\u3051\u305f\u307e\u307e\u3001\u9178\u6027\u6db2\u3092\u6ef4\u4e0b\u3057\u3066\u3044\u308b\u65b9\u6cd5\u306f\u3001\u4e39\u7fbd\u6c0f\u4ee5\u5916\u306e\u4ed6\u306e\u5b9f\u9a13\u8005\u306f\u3084\u3063\u3066\u3044\u306a\u3044\u3067\u3057\u3087\u3046\u3002\u300d<\/span><\/a><\/p>\n

STAP\u7d30\u80de\u4f5c\u6210\u30d7\u30ed\u30c8\u30b3\u30eb\u304c\uff14\u3064\u3082\u3042\u308b\u306e\u306f\u7570\u5e38\u3060\u3068\u3059\u3067\u306b\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u305d\u306e\u901a\u308a\u3067\u3001\u3064\u307e\u308a\u9178\u6d74\u7d30\u80de\u306f\u8ad6\u6587\u901a\u308a\u3067\u304d\u306a\u3044\u3053\u3068\u3092\u8457\u8005\u7b49\u304c\u767d\u72b6\u3057\u3066\u3044\u308b\u3088\u3046\u306a\u3082\u306e\u3067\u3059\u3002<\/p>\n

\u5b66\u3068\u307f\u5b50\u306f\u5c0f\u4fdd\u65b9\u6c0f\u3084\u4e39\u7fbd\u6c0f\u306f\u300c\u5206\u5316\u7d30\u80de\u3092\u57f9\u990a\u6db2\u306b\u3064\u3051\u305f\u307e\u307e\u3001\u9178\u6027\u6db2\u3092\u6ef4\u4e0b\u3057\u3066\u3044\u308b\u65b9\u6cd5\u300d\u3060\u3068\u3044\u3046\u306e\u3067\u3059\u304c\u305d\u3046\u3067\u3057\u3087\u3046\u304b\u3002\u6539\u3081\u3066\u30c1\u30a7\u30c3\u30af\u3057\u3066\u307f\u307e\u3059\u3002
\n1\ufe0f\u20e3Nature Article
\nAfter cell sorting, 1 X10^6 CD45-positive cells were treated with 500 \u00b5l of low-pH HBSS solution (titrated to pH5.7 by HCl) for 25 min at 37 uC, and then centrifuged at 1,000 r.p.m. at room temperature for 5 min.\u7d30\u80de\u9078\u5225\u5f8c\u30011 X10^6\u500b\u306eCD45\u967d\u6027\u7d30\u80de\u3092\u3001\u5869\u9178\u3067pH5.7\u306b\u8abf\u6574\u3057\u305f\u4f4epH HBSS\u6eb6\u6db2500 ml\u4e2d\u306737\u2103\u306b\u306625\u5206\u9593\u51e6\u7406\u3057\u3001\u305d\u306e\u5f8c\u3001\u5ba4\u6e29\u30671,000 rpm\u306b\u30665\u5206\u9593\u9060\u5fc3\u5206\u96e2\u3057\u305f\u3002
\n2\ufe0f\u20e3\u4e39\u7fbd\u30d7\u30ed\u30c8\u30b3\u30ebhttps:\/\/www.protocols.io\/view\/essential-technical-tips-for-stap-cell-conversion-36wgqdz93vk5\/v1
\nAfter cell sorting, 1\u2009\u00d7 10^6 CD45+ cells were treated with 500\u2009 \u00b5l of low-pH HBSS solution (titrated to pH 5.7 by HCl) for 25\u2009min at 37\u00b0C, and then centrifuged at 1,000\u2009rpm at room temperature for 5\u2009min.\u3000\u7d30\u80de\u9078\u5225\u5f8c\u30011\u00d710\u2076\u500b\u306eCD45+\u7d30\u80de\u3092\u3001500 \u00b5l\u306e\u4f4epH HBSS\u6eb6\u6db2\uff08\u5869\u9178\u3067pH 5.7\u306b\u8abf\u6574\u3057\u305f\u3082\u306e\uff09\u306b37\u00b0C\u306725\u5206\u9593\u51e6\u7406\u3057\u3001\u305d\u306e\u5f8c\u3001\u5ba4\u6e29\u30671,000 rpm\u30675\u5206\u9593\u9060\u5fc3\u5206\u96e2\u3057\u305f\u3002
\n3\ufe0f\u20e3Vacanti\u30d7\u30ed\u30c8\u30b3\u30ebhttps:\/\/web.archive.org\/web\/20140321094008\/http:\/\/research.bwhanesthesia.org\/research-groups\/cterm\/stap-cell-protocol
\n15 mL\u30c1\u30e5\u30fc\u30d6\u306bHBSS\u30925 mL\u5165\u308c\u308b\u3002 Hanks Balanced Saline Solution\uff08HBSS Ca+Mg+ Free\uff1aGibco 14170-112\uff09 \u306b\u6e05\u6f54\u306apH\u30bb\u30f3\u30b5\u30fc\u3092\u633f\u5165\u3059\u308b\u3002ATP\u30b9\u30c8\u30c3\u30af\u6eb6\u6db2\uff08\u307b\u307c\u98fd\u548c\u306eATP\u6eb6\u6db2\uff09\u3092HBSS\u30781\u6ef4\u305a\u3064\u6ef4\u4e0b\u3057\u3066\u6ef4\u5b9a\u3057\u3001\u76ee\u7684\u306epH 5.0\u306b\u5230\u9054\u3059\u308b\u307e\u3067\u8abf\u6574\u3059\u308b\uff08\u76ee\u5b89\uff1a\u6570\u767e\u00b5L\u7a0b\u5ea6\uff09\u3002\u6e2c\u5b9a\u3092\u6b63\u78ba\u306b\u3059\u308b\u305f\u3081\u3001\u9069\u5b9c\u6eb6\u6db2\u3092\u6df7\u548c\u3059\u308b\u3002\u6700\u7d42\u7684\u306b\u5f97\u3089\u308c\u308b\u9178\u6027HBSS\u6eb6\u6db2\u4e2d\u306eATP\u6fc3\u5ea6\u306f\u7d043\uff5e5 mM\u3068\u306a\u308b\u3002\u3053\u3053\u306b\u9060\u5fc3\u3057\u3066\u96c6\u3081\u305f\u7d30\u80de\u3092\u3044\u308c\u7d30\u80de\u61f8\u6fc1\u6db2\u3092\u30d4\u30da\u30c3\u30c8\u30675\u5206\u9593\u3001\u5438\u5f15\u30fb\u5410\u51fa\uff08\u30c8\u30ea\u30c1\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\uff09\u3059\u308b\u3002
\n4\ufe0f\u20e3\u5c0f\u4fdd\u65b9\u30d7\u30ed\u30c8\u30b3\u30ebSTAP HOPE PAGE https:\/\/web.archive.org\/web\/20160331100934\/https:\/\/stap-hope-page.com\/protocol-for-stap-cells
\nAdd 500ml of HBSS and suspend a cell pellet using a 1000\u03bcl-pipette*** Add 6\u03bcl of ATP solution \uff08\u307b\u307c\u98fd\u548c\u306eATP\u6db2\uff09(At this moment, the color of HBSS changes from red to yellow) Incubate the cells horizontally at 37\u2103 (in the 5% CO2 incubator) for 15 min**** (During this time, count the number of cells)\u3068\u3042\u3063\u3066\u3001pH\u3092\u6e2c\u5b9a\u3057\u3066\u3044\u306a\u3044\u3002<\/p>\n

\u201c500 \u00b5l of low-pH HBSS solution (titrated to pH 5.7 by HCl)\u201d\u306f\u300c\u4f4e pH \u306e HBSS \u6eb6\u6db2\u300d\u3092\u4f5c\u3063\u3066\u304a\u304d\u3001\u305d\u3053\u3078 CD45+ \u7d30\u80de\uff081\u00d710^6 cells\uff09\u3092\u52a0\u3048\u306625\u5206\u9593 37\u00b0C \u3067\u51e6\u7406\u3057\u305f\u3001\u3068\u3044\u3046\u3053\u3068\uff08\u4e0b\u8a18\uff0a\uff09\u3067\u3001\u5b66\u3068\u307f\u5b50\u306e\u8a00\u3046\u3088\u3046\u306a\u7d30\u80de\u304c\u5165\u3063\u3066\u3044\u308b\u57f9\u990a\u6db2\u306b\u5869\u9178\u3092\u6ef4\u4e0b\u3057\u3066pH\u30925.7\u306b\u3057\u305f\u306e\u3067\u306f\u306a\u3044\u306e\u3067\u3059\u3002<\/p>\n

\u3064\u307e\u308a\u5b66\u3068\u307f\u5b50\u306e\u8a00\u3046\u300c\u5206\u5316\u7d30\u80de\u3092\u57f9\u990a\u6db2\u306b\u3064\u3051\u305f\u307e\u307e\u3001\u9178\u6027\u6db2\u3092\u6ef4\u4e0b\u300d<\/span>\u3068\u3044\u3046\u30d7\u30ed\u30c8\u30b3\u30eb\u306f\u3069\u3053\u306b\u3082\u306a\u3044\u306e\u3067\u3059\u3002\u5b66\u3068\u307f\u5b50\u306f(titrated to pH 5.7 by HCl)\u3092\u8aad\u307f\u9055\u3048\u3066\u3044\u308b\u306e\u3067\u3059\u3002<\/p>\n

\uff1e\u5b66\u3068\u307f\u5b50
\n\u300c\u5206\u5316\u7d30\u80de\u3092\u57f9\u990a\u6db2\u306b\u3064\u3051\u305f\u307e\u307e\u3001\u9178\u6027\u6db2\u3092\u6ef4\u4e0b\u300d<\/span>\u3068\u3044\u3046\u306e\u306f\u3069\u3053\u306b\u66f8\u3044\u3066\u3042\u308b\u65b9\u6cd5\u306a\u3093\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n

\uff0a<\/p>\n

Q\uff1a\u3000After cell sorting, 1\u2009\u00d7 10^6 CD45+ cells were treated with 500\u2009 \u00b5l of low-pH HBSS solution (titrated to pH 5.7 by HCl) for 25\u2009min at 37\u00b0C, and then centrifuged at 1,000\u2009rpm at room temperature for 5\u2009min.\u3000\u3068\u3044\u3046\u65b9\u6cd5\u306f\u300c\u3042\u3089\u304b\u3058\u3081\u5869\u9178\u3092\u6ef4\u4e0b\u3057\u3066pH\u30925.7 \u306b\u3057\u305f HBSS\u6db2\u306b\u7d30\u80de\u3092\u5165\u308c\u305f\u300d\u3068\u300c\u7d30\u80de\u306e\u5165\u3063\u305fHBSS\u6db2\u306b\u5869\u9178\u3092\u6ef4\u4e0b\u3057\u3066\uff50H\u30925.7\u306b\u3057\u305f\u300d\u306e\u3069\u3061\u3089\u3067\u3059\u304b\uff1f<\/p>\n

ChatgGPT\uff1a\u3000\u3053\u306e\u82f1\u6587\u306e\u89e3\u91c8\u3068\u3057\u3066\u306f\u3001
\n> \u201c500 \u00b5l of low-pH HBSS solution (titrated to pH 5.7 by HCl)\u201d
\n\u3068\u306a\u3063\u3066\u3044\u308b\u306e\u3067\u3001
\n**\u300c\u3042\u3089\u304b\u3058\u3081\u5869\u9178\uff08HCl\uff09\u3067 pH 5.7 \u306b\u8abf\u6574\u3057\u3066\u304a\u3044\u305f HBSS \u6eb6\u6db2\u3092\u7528\u610f\u3057\u3001\u305d\u306e\u4f4epH HBSS\u306b\u7d30\u80de\u3092\u5165\u308c\u3066\u51e6\u7406\u3057\u305f\u300d**
\n\u3068\u3044\u3046\u610f\u5473\u3067\u3059\u3002
\n\u3064\u307e\u308a\u3001\u5f8c\u8005\u306e
\n> \u300c\u7d30\u80de\u306e\u5165\u3063\u305fHBSS\u6db2\u306b\u5869\u9178\u3092\u6ef4\u4e0b\u3057\u3066 pH 5.7 \u306b\u3057\u305f\u300d
\n\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002
\n\u7406\u7531\u3068\u3057\u3066\u306f\u3001\u82f1\u8a9e\u3067 \u201cHBSS solution (titrated to pH 5.7 by HCl)\u201d \u304c HBSS \u6eb6\u6db2\u81ea\u4f53\u3092\u4fee\u98fe\u3057\u3066\u304a\u308a\u3001\u300cHBSS \u3092 HCl \u3067 pH 5.7 \u306b\u8abf\u6574\u3057\u305f\u6eb6\u6db2\u300d\u3068\u3044\u3046\u69cb\u9020\u306b\u306a\u3063\u3066\u3044\u308b\u305f\u3081\u3067\u3059\u3002
\n\u5b9f\u9a13\u64cd\u4f5c\u3068\u3057\u3066\u3082\u3001\u901a\u5e38\u306f\uff1a
\n1. HBSS \u3092\u5225\u5bb9\u5668\u3067 HCl \u306b\u3088\u308a pH 5.7 \u306b\u8abf\u6574
\n2. \u305d\u306e low-pH HBSS \u3092\u7d30\u80de\u3078\u6dfb\u52a0
\n3. 37\u00b0C\u306725\u5206\u51e6\u7406
\n\u3068\u3044\u3046\u6d41\u308c\u306b\u306a\u308a\u307e\u3059\u3002\u7d30\u80de\u61f8\u6fc1\u6db2\u306b\u76f4\u63a5 HCl \u3092\u6ef4\u4e0b\u3059\u308b\u3068\u3001\u5c40\u6240\u7684\u306a\u6025\u6fc0\u306apH\u4f4e\u4e0b\u304c\u8d77\u304d\u3084\u3059\u304f\u3001\u518d\u73fe\u6027\u3082\u60aa\u304f\u306a\u308b\u305f\u3081\u3001\u4e00\u822c\u7684\u306a\u8a18\u8f09\u6cd5\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\n<\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"

\u4ee5\u524d\u304b\u3089\u601d\u3063\u3066\u3044\u305f\u306e\u3067\u3059\u304c\u3001\u76db\u3093\u306b\u5b66\u3068\u307f\u5b50\u306f\u9178\u6d74\u306e\u65b9\u6cd5\u304c\u3001\u4ed6\u306e\u518d\u73fe\u5b9f\u9a13\u3092\u884c\u3063\u305f\u7814\u7a76\u8005\u306f\u518d\u73fe\u3057\u3066\u3044\u306a\u3044\u3068\u3044\u3046\u308f\u3051\u3067\u3059\u3002 \u300c\u5206\u5316\u7d30\u80de\u3092\u57f9\u990a\u6db2\u306b\u3064\u3051\u305f\u307e\u307e\u3001\u9178\u6027\u6db2\u3092\u6ef4\u4e0b\u3057\u3066\u3044\u308b\u65b9\u6cd5\u306f\u3001\u4e39\u7fbd\u6c0f\u4ee5\u5916\u306e\u4ed6\u306e\u5b9f\u9a13\u8005\u306f\u3084\u3063\u3066\u3044\u306a\u3044\u3067\u3057\u3087 … \u7d9a\u304d\u3092\u8aad\u3080 STAP\u7d30\u80de\uff14\u3064\u306e\u30d7\u30ed\u30c8\u30b3\u30eb<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2,8],"tags":[],"class_list":["post-28247","post","type-post","status-publish","format-standard","hentry","category-education","category-fake"],"_links":{"self":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/28247","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28247"}],"version-history":[{"count":7,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/28247\/revisions"}],"predecessor-version":[{"id":28250,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/28247\/revisions\/28250"}],"wp:attachment":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28247"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28247"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28247"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}