{"id":28247,"date":"2026-05-26T17:49:17","date_gmt":"2026-05-26T08:49:17","guid":{"rendered":"https:\/\/nbsigh2.com\/?p=28247"},"modified":"2026-05-31T05:50:21","modified_gmt":"2026-05-30T20:50:21","slug":"stap%e7%b4%b0%e8%83%9e%ef%bc%94%e3%81%a4%e3%81%ae%e3%83%97%e3%83%ad%e3%83%88%e3%82%b3%e3%83%ab","status":"publish","type":"post","link":"https:\/\/nbsigh2.com\/?p=28247","title":{"rendered":"STAP\u7d30\u80de\uff14\u3064\u306e\u30d7\u30ed\u30c8\u30b3\u30eb"},"content":{"rendered":"

\u4ee5\u524d\u304b\u3089\u601d\u3063\u3066\u3044\u305f\u306e\u3067\u3059\u304c\u3001\u76db\u3093\u306b\u5b66\u3068\u307f\u5b50\u306f\u9178\u6d74\u306e\u65b9\u6cd5\u304c\u3001\u4ed6\u306e\u518d\u73fe\u5b9f\u9a13\u3092\u884c\u3063\u305f\u7814\u7a76\u8005\u306f\u518d\u73fe\u3057\u3066\u3044\u306a\u3044\u3068\u3044\u3046\u308f\u3051\u3067\u3059\u3002
\n\uff08\uff12\uff16\u65e5\u5915\u65b9\u6700\u521d\u306b\u30a2\u30c3\u30d7\u3057\u305f\u5185\u5bb9\u3092\uff12\uff17\u65e5\u65e9\u671d\u4e00\u90e8\u5909\u66f4\u3057\u307e\u3057\u305f\uff09<\/p>\n

\u300c\u5206\u5316\u7d30\u80de\u3092\u57f9\u990a\u6db2\u306b\u3064\u3051\u305f\u307e\u307e\u3001\u9178\u6027\u6db2\u3092\u6ef4\u4e0b\u3057\u3066\u3044\u308b\u65b9\u6cd5\u306f\u3001\u4e39\u7fbd\u6c0f\u4ee5\u5916\u306e\u4ed6\u306e\u5b9f\u9a13\u8005\u306f\u3084\u3063\u3066\u3044\u306a\u3044\u3067\u3057\u3087\u3046\u3002\u5c0f\u4fdd\u65b9\u6c0f\u304c\u7de8\u307f\u51fa\u3057\u305f\u65b9\u6cd5\u306a\u3093\u3067\u3057\u3087\u3046\u306d\u3002\u3057\u304b\u3057\u3001\u4ed6\u306e\u518d\u73fe\u5b9f\u9a13\u3067\u306f\u3084\u3089\u308c\u3066\u3044\u307e\u305b\u3093\u3002\u300d<\/span><\/a><\/p>\n

STAP\u7d30\u80de\u4f5c\u6210\u30d7\u30ed\u30c8\u30b3\u30eb\u304c\uff14\u3064\u3082\u3042\u308b\u306e\u306f\u7570\u5e38\u3060\u3068\u3059\u3067\u306b\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u305d\u306e\u901a\u308a\u3067\u3001\u3064\u307e\u308a\u9178\u6d74\u7d30\u80de\u306f\u8ad6\u6587\u901a\u308a\u3067\u304d\u306a\u3044\u3053\u3068\u3092\u8457\u8005\u7b49\u304c\u767d\u72b6\u3057\u3066\u3044\u308b\u3088\u3046\u306a\u3082\u306e\u3067\u3059\u3002<\/p>\n

\u5b66\u3068\u307f\u5b50\u306f\u5c0f\u4fdd\u65b9\u6c0f\u3084\u4e39\u7fbd\u6c0f\u306f\u300c\u5206\u5316\u7d30\u80de\u3092\u57f9\u990a\u6db2\u306b\u3064\u3051\u305f\u307e\u307e\u3001\u9178\u6027\u6db2\u3092\u6ef4\u4e0b\u3057\u3066\u3044\u308b\u65b9\u6cd5\u300d<\/span>\u3060\u3068\u3044\u3046\u306e\u3067\u3059\u304c\u305d\u3046\u3067\u3057\u3087\u3046\u304b\u3002\u6539\u3081\u3066\u30c1\u30a7\u30c3\u30af\u3057\u3066\u307f\u307e\u3059\u3002
\n1\ufe0f\u20e3Nature Article
\nAfter cell sorting, 1 X10^6 CD45-positive cells were treated with 500 \u00b5l of low-pH HBSS solution (titrated to pH5.7 by HCl) for 25 min at 37 \u2103, and then centrifuged at 1,000 r.p.m. at room temperature for 5 min.
\n\u7d30\u80de\u9078\u5225\u5f8c\u3001CD45\u967d\u6027\u7d30\u80de1\u00d710^6\u500b\u3092\u3001\u5869\u9178\u3067pH5.7\u306b\u8abf\u6574\u3057\u305f\u4f4epH HBSS\u6eb6\u6db2500 \u00b5l\u4e2d\u306737\u2103\u306b\u306625\u5206\u9593\u51e6\u7406\u3057\u3001\u305d\u306e\u5f8c\u3001\u5ba4\u6e29\u30671,000 r.p.m.\u306b\u30665\u5206\u9593\u9060\u5fc3\u5206\u96e2\u3057\u305f(DeepL\u8a33\uff09\u3002
\n2\ufe0f\u20e3\u4e39\u7fbd\u30d7\u30ed\u30c8\u30b3\u30ebhttps:\/\/www.protocols.io\/view\/essential-technical-tips-for-stap-cell-conversion-36wgqdz93vk5\/v1<\/a>
\n5 After cell sorting, 1\u2009\u00d7 10^6 CD45+ cells were treated with 500\u2009 \u00b5l of low-pH HBSS solution (titrated to pH 5.7 by HCl) for 25\u2009min at 37\u00b0C, and then centrifuged at 1,000\u2009rpm at room temperature for 5\u2009min.\u3000
\n\u7d30\u80de\u9078\u5225\u5f8c\u30011\u00d710\u2076\u500b\u306eCD45+\u7d30\u80de\u3092\u3001500 \u00b5l\u306e\u4f4epH HBSS\u6eb6\u6db2\uff08\u5869\u9178\u3067pH 5.7\u306b\u8abf\u6574\u3057\u305f\u3082\u306e\uff09\u306b37\u00b0C\u306725\u5206\u9593\u51e6\u7406\u3057\u3001\u305d\u306e\u5f8c\u3001\u5ba4\u6e29\u30671,000 rpm\u30675\u5206\u9593\u9060\u5fc3\u5206\u96e2\u3057\u305f(DeepL\u8a33\uff09\u3002
\n5.1 The buffering action of HBSS is weak, so carry-over of the solution may affect pH. Please adjust pH to 5.7 in cell suspension by the following method. First, suspend the cell pellet with 494 \u00b5l of HBSS pre-chilled at 4\u00b0C, then add 6 \u00b5l of diluted HCl (10 \u00b5l of 35% HCl in 590 \u00b5l of HBSS) to adjust to a final pH of 5.7. Please confirm the final pH in a pilot experiment, and optimize the volume of HCl added, as necessary. Alternatively, suspend the cell pellet in HBSS-pH 5.4 pre-chilled at 4\u00b0C.
\nHBSS\u306e\u7de9\u885d\u4f5c\u7528\u306f\u5f31\u3044\u305f\u3081\u3001\u6eb6\u6db2\u306e\u6301\u3061\u8d8a\u3057\u304cpH\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u4ee5\u4e0b\u306e\u65b9\u6cd5\u306b\u5f93\u3063\u3066\u3001\u7d30\u80de\u61f8\u6fc1\u6db2\u306epH\u30925.7\u306b\u8abf\u6574\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u307e\u305a\u30014\u00b0C\u3067\u4e88\u51b7\u3057\u305fHBSS 494 \u00b5l\u3067\u7d30\u80de\u30da\u30ec\u30c3\u30c8\u3092\u61f8\u6fc1\u3055\u305b\u3001\u6b21\u306b\u5e0c\u91c8\u3057\u305fHCl\uff0835% HCl 10 \u00b5l\u3092HBSS 590 \u00b5l\u306b\u6eb6\u89e3\u3057\u305f\u3082\u306e\uff096 \u00b5l\u3092\u52a0\u3048\u3066\u3001\u6700\u7d42pH\u30925.7\u306b\u8abf\u6574\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u4e88\u5099\u5b9f\u9a13\u3067\u6700\u7d42pH\u3092\u78ba\u8a8d\u3057\u3001\u5fc5\u8981\u306b\u5fdc\u3058\u3066HCl\u306e\u6dfb\u52a0\u91cf\u3092\u6700\u9069\u5316\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3042\u308b\u3044\u306f\u30014\u00b0C\u3067\u4e88\u51b7\u3057\u305fpH 5.4\u306eHBSS\u3092\u7528\u3044\u3066\u7d30\u80de\u30da\u30ec\u30c3\u30c8\u3092\u61f8\u6fc1\u3055\u305b\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059(DeepL\u8a33\uff09\u3002
\n3\ufe0f\u20e3Vacanti\u30d7\u30ed\u30c8\u30b3\u30ebhttps:\/\/web.archive.org\/web\/20140321094008\/http:\/\/research.bwhanesthesia.org\/research-groups\/cterm\/stap-cell-protocol<\/a>
\nA6. Add HBSS to the suspension to a total volume of 20ml, centrifuge at 1200rpm for 5 minutes and then aspirate the supernatant.
\nA7. Resuspend the cells in HBSS at a pH of 5.4, at cell concentration of 2 million cells\/ml, then place in an incubator at 37\u00b0C for 25 minutes.
\nA6. \u61f8\u6fc1\u6db2\u306bHBSS\u3092\u52a0\u3048\u3066\u7dcf\u5bb9\u91cf\u309220ml\u3068\u3057\u30011200rpm\u30675\u5206\u9593\u9060\u5fc3\u5206\u96e2\u3057\u305f\u5f8c\u3001\u4e0a\u6e05\u3092\u5438\u5f15\u3059\u308b\u3002
\nA7. \u7d30\u80de\u3092pH 5.4\u306eHBSS\u3067200\u4e07\u7d30\u80de\/ml\u306e\u6fc3\u5ea6\u306b\u306a\u308b\u3088\u3046\u306b\u518d\u61f8\u6fc1\u3057\u300137\u00b0C\u306e\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u306725\u5206\u9593\u57f9\u990a\u3059\u308b(DeepL\u8a33\uff09\u3002<\/p>\n

4\ufe0f\u20e3\u5c0f\u4fdd\u65b9\u30d7\u30ed\u30c8\u30b3\u30ebSTAP HOPE PAGE https:\/\/archive.md\/cJifN<\/a>
\n11. Add 500\u03bcl of HBSS and suspend a cell pellet using a 1000\u03bcl-pipette***
\n12. Take out 6\u03bcl of the cell suspension for cell counting
\n13. Add 6\u03bcl of ATP solution\uff08\u307b\u307c\u98fd\u548c\u306eATP\u6db2\uff09 (At this moment, the color of HBSS changes from red to yellow)
\n14. Incubate the cells horizontally at 37\u2103 (in the 5% CO2 incubator) for 15 min**** (During this time, count the number of cells)
\n11. HBSS\u3092500\u03bcl\u52a0\u3048\u30011000\u03bcl\u30d4\u30da\u30c3\u30c8\u3092\u7528\u3044\u3066\u7d30\u80de\u30da\u30ec\u30c3\u30c8\u3092\u61f8\u6fc1\u3055\u305b\u308b***
\n12. \u7d30\u80de\u61f8\u6fc1\u6db2\u304b\u30896\u03bcl\u3092\u63a1\u53d6\u3057\u3001\u7d30\u80de\u6570\u3092\u6e2c\u5b9a\u3059\u308b
\n13. ATP\u6eb6\u6db2\uff08\u307b\u307c\u98fd\u548c\u306eATP\u6db2\uff09\u30926\u03bcl\u52a0\u3048\u308b\uff08\u3053\u306e\u6642\u70b9\u3067\u3001HBSS\u306e\u8272\u304c\u8d64\u304b\u3089\u9ec4\u8272\u306b\u5909\u5316\u3059\u308b\uff09
\n14. \u7d30\u80de\u3092\u6c34\u5e73\u306b\u4fdd\u3061\u300137\u2103\uff085% CO\u2082\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u5185\uff09\u306715\u5206\u9593\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30c8\u3059\u308b****\uff08\u3053\u306e\u9593\u306b\u7d30\u80de\u6570\u3092\u8a08\u6570\u3059\u308b\uff09
\n\u3068\u3042\u3063\u3066\u3001pH\u3092\u6e2c\u5b9a\u3057\u3066\u3044\u306a\u3044\u3002<\/p>\n

\u8ad6\u6587\u3001\u4e39\u7fbd\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u201c500 \u00b5l of low-pH HBSS solution (titrated to pH 5.7 by HCl)\u201d\u306f\u300c\u4f4e pH \u306e HBSS \u6eb6\u6db2\u300d\u3092\u4f5c\u3063\u3066\u304a\u304d\u3001\u305d\u3053\u3078 CD45+ \u7d30\u80de\uff081\u00d710^6 cells\uff09\u3092\u52a0\u3048\u306625\u5206\u9593 37\u00b0C \u3067\u51e6\u7406\u3057\u305f\u3001\u3068\u3044\u3046\u3053\u3068\uff08\u4e0b\u8a18\uff0a\uff09\u3067\u3001\u5b66\u3068\u307f\u5b50\u306e\u8a00\u3046\u3088\u3046\u306a\u7d30\u80de\u304c\u5165\u3063\u3066\u3044\u308b\u57f9\u990a\u6db2\u306b\u5869\u9178\u3092\u6ef4\u4e0b\u3057\u3066pH\u30925.7\u306b\u3057\u305f\u306e\u3067\u306f\u306a\u3044\u306e\u3067\u3059\u3002\u4e39\u7fbd\u6c0f\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u306f\u8ad6\u6587\u3068\u4e00\u81f4\u3057\u3066\u3044\u307e\u3059\u304c\u30015.1 \u3068\u3057\u3066\u88dc\u8db3\uff1f\u306b\u7d30\u80de\u61f8\u6fc1\u6db2\u306b\u5e0c\u91c8\u5869\u9178\u3092\u52a0\u3048\u308b\u65b9\u6cd5\u3068\u3001\u3042\u3089\u304b\u3058\u3081\u9178\u6027\u306b\u3057\u305f\u51b7\u3084\u3057\u305f\u57f9\u990a\u6db2\u306b\u7d30\u80de\u3092\u52a0\u3048\u308b\u65b9\u6cd5\u3082\u66f8\u3044\u3066\u3042\u308a\u307e\u3059\u3002\uff13\u7a2e\u985e\u3082\u3042\u3063\u3066\u3001\u3069\u306e\u65b9\u6cd5\u304c\u3044\u3044\u306e\u304b\u66f8\u3044\u3066\u306a\u3044\u3068\u3044\u3046\u304a\u7c97\u672b\u306a\u3082\u306e\u3067\u3001\u3053\u308c\u306f\u4e39\u7fbd\u6c0f\u81ea\u8eab\u304c\u5b9f\u65bd\u3057\u305f\u306e\u3067\u306f\u306a\u304f\u3001\u5c0f\u4fdd\u65b9\u6c0f\u304b\u3089\u805e\u304d\u53d6\u3063\u3066\u66f8\u3044\u305f\u305f\u3081\u306e\u3088\u3046\u3067\u3059\u3002\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u5b9f\u969b\u306b\u3084\u3063\u305f\u3053\u3068\u306e\u306a\u3044\u65b9\u304c\u66f8\u304f\u3068\u3044\u3046\u306e\u306f\u3042\u307e\u308a\u306b\u3082\u304a\u7c97\u672b\u3067\u3059\u3002<\/p>\n

\u3064\u307e\u308a\u5b66\u3068\u307f\u5b50\u306e\u8a00\u3046\u300c\u5206\u5316\u7d30\u80de\u3092\u57f9\u990a\u6db2\u306b\u3064\u3051\u305f\u307e\u307e\u3001\u9178\u6027\u6db2\u3092\u6ef4\u4e0b\u300d<\/span>\u3068\u3044\u3046\u30d7\u30ed\u30c8\u30b3\u30eb\u306f\u57fa\u672c\u7684\u306b\u306a\u3044\u306e\u3067\u3059\u3002\u5b66\u3068\u307f\u5b50\u306f(titrated to pH 5.7 by HCl)\u3092\u8aad\u307f\u9055\u3048\u3066\u3044\u308b\u306e\u3067\u3059\u3002\u7d30\u80de\u61f8\u6fc1\u6db2\u306b\u5869\u9178\u3042\u308b\u3044\u306fATP\u3092\u6ef4\u4e0b\u3059\u308b\u3068\u3044\u3046\u306e\u306f\u3001\u5b9f\u65bd\u3057\u305f\u306e\u304b\u7591\u308f\u3057\u3044\u4e39\u7fbd\u6c0f\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u4e00\u90e8\u3068\u5c0f\u4fdd\u65b9\u6c0f\u306e\u30d7\u30e9\u30a4\u30d9\u30fc\u30c8\u306aWeb\u30da\u30fc\u30b8\u306b\u3042\u308b\u8ad6\u6587\u8a18\u8f09\u3068\u9055\u3046\u3082\u306e\u3060\u3051\u3067\u3001\u3069\u308c\u304cSTAP\u7d30\u80de\u4f5c\u6210\u306b\u6700\u9069\u306a\u30d7\u30ed\u30c8\u30b3\u30eb\u306a\u306e\u304b\u308f\u304b\u3089\u306a\u3044\u306e\u3067\u3059\u3002\u3044\u305a\u308c\u306b\u3057\u308d\u3001\u8ad6\u6587\u306b\u3042\u308b\u3088\u3046\u306a\u91cf\u306e\u7d30\u80de\u306f\u3001\u5c0f\u4fdd\u65b9\u6c0f\u81ea\u8eab\u3067\u3059\u3089\u3001\u3067\u304d\u306a\u304b\u3063\u305f\u306e\u3067\u610f\u5473\u306f\u306a\u3044\u306e\u3067\u3059\u3051\u3069\u306d\u3002<\/p>\n

\uff1e\u5b66\u3068\u307f\u5b50
\n\u300c\u5206\u5316\u7d30\u80de\u3092\u57f9\u990a\u6db2\u306b\u3064\u3051\u305f\u307e\u307e\u3001\u9178\u6027\u6db2\u3092\u6ef4\u4e0b\u300d<\/span>\u3068\u3044\u3046\u306e\u306f\u3069\u3053\u306b\u66f8\u3044\u3066\u3042\u308b\u65b9\u6cd5\u3092\u8aad\u3093\u3067\u767a\u8a00\u3057\u3066\u3044\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u5f53\u65b9\u304c\u6319\u3052\u305f\u4ee5\u5916\u306b\u3042\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n

\uff0a<\/p>\n

Q\uff1a\u3000After cell sorting, 1\u2009\u00d7 10^6 CD45+ cells were treated with 500\u2009 \u00b5l of low-pH HBSS solution (titrated to pH 5.7 by HCl) for 25\u2009min at 37\u00b0C, and then centrifuged at 1,000\u2009rpm at room temperature for 5\u2009min.\u3000\u3068\u3044\u3046\u65b9\u6cd5\u306f\u300c\u3042\u3089\u304b\u3058\u3081\u5869\u9178\u3092\u6ef4\u4e0b\u3057\u3066pH\u30925.7 \u306b\u3057\u305f HBSS\u6db2\u306b\u7d30\u80de\u3092\u5165\u308c\u305f\u300d\u3068\u300c\u7d30\u80de\u306e\u5165\u3063\u305fHBSS\u6db2\u306b\u5869\u9178\u3092\u6ef4\u4e0b\u3057\u3066\uff50H\u30925.7\u306b\u3057\u305f\u300d\u306e\u3069\u3061\u3089\u3067\u3059\u304b\uff1f<\/p>\n

ChatgGPT\uff1a\u3000\u3053\u306e\u82f1\u6587\u306e\u89e3\u91c8\u3068\u3057\u3066\u306f\u3001
\n> \u201c500 \u00b5l of low-pH HBSS solution (titrated to pH 5.7 by HCl)\u201d
\n\u3068\u306a\u3063\u3066\u3044\u308b\u306e\u3067\u3001
\n**\u300c\u3042\u3089\u304b\u3058\u3081\u5869\u9178\uff08HCl\uff09\u3067 pH 5.7 \u306b\u8abf\u6574\u3057\u3066\u304a\u3044\u305f HBSS \u6eb6\u6db2\u3092\u7528\u610f\u3057\u3001\u305d\u306e\u4f4epH HBSS\u306b\u7d30\u80de\u3092\u5165\u308c\u3066\u51e6\u7406\u3057\u305f\u300d**
\n\u3068\u3044\u3046\u610f\u5473\u3067\u3059\u3002
\n\u3064\u307e\u308a\u3001\u5f8c\u8005\u306e
\n> \u300c\u7d30\u80de\u306e\u5165\u3063\u305fHBSS\u6db2\u306b\u5869\u9178\u3092\u6ef4\u4e0b\u3057\u3066 pH 5.7 \u306b\u3057\u305f\u300d
\n\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002
\n\u7406\u7531\u3068\u3057\u3066\u306f\u3001\u82f1\u8a9e\u3067 \u201cHBSS solution (titrated to pH 5.7 by HCl)\u201d \u304c HBSS \u6eb6\u6db2\u81ea\u4f53\u3092\u4fee\u98fe\u3057\u3066\u304a\u308a\u3001\u300cHBSS \u3092 HCl \u3067 pH 5.7 \u306b\u8abf\u6574\u3057\u305f\u6eb6\u6db2\u300d\u3068\u3044\u3046\u69cb\u9020\u306b\u306a\u3063\u3066\u3044\u308b\u305f\u3081\u3067\u3059\u3002
\n\u5b9f\u9a13\u64cd\u4f5c\u3068\u3057\u3066\u3082\u3001\u901a\u5e38\u306f\uff1a
\n1. HBSS \u3092\u5225\u5bb9\u5668\u3067 HCl \u306b\u3088\u308a pH 5.7 \u306b\u8abf\u6574
\n2. \u305d\u306e low-pH HBSS \u3092\u7d30\u80de\u3078\u6dfb\u52a0
\n3. 37\u00b0C\u306725\u5206\u51e6\u7406
\n\u3068\u3044\u3046\u6d41\u308c\u306b\u306a\u308a\u307e\u3059\u3002\u7d30\u80de\u61f8\u6fc1\u6db2\u306b\u76f4\u63a5 HCl \u3092\u6ef4\u4e0b\u3059\u308b\u3068\u3001\u5c40\u6240\u7684\u306a\u6025\u6fc0\u306apH\u4f4e\u4e0b\u304c\u8d77\u304d\u3084\u3059\u304f\u3001\u518d\u73fe\u6027\u3082\u60aa\u304f\u306a\u308b\u305f\u3081\u3001\u4e00\u822c\u7684\u306a\u8a18\u8f09\u6cd5\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\n<\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"

\u4ee5\u524d\u304b\u3089\u601d\u3063\u3066\u3044\u305f\u306e\u3067\u3059\u304c\u3001\u76db\u3093\u306b\u5b66\u3068\u307f\u5b50\u306f\u9178\u6d74\u306e\u65b9\u6cd5\u304c\u3001\u4ed6\u306e\u518d\u73fe\u5b9f\u9a13\u3092\u884c\u3063\u305f\u7814\u7a76\u8005\u306f\u518d\u73fe\u3057\u3066\u3044\u306a\u3044\u3068\u3044\u3046\u308f\u3051\u3067\u3059\u3002 \uff08\uff12\uff16\u65e5\u5915\u65b9\u6700\u521d\u306b\u30a2\u30c3\u30d7\u3057\u305f\u5185\u5bb9\u3092\uff12\uff17\u65e5\u65e9\u671d\u4e00\u90e8\u5909\u66f4\u3057\u307e\u3057\u305f\uff09 \u300c\u5206\u5316\u7d30\u80de\u3092\u57f9\u990a\u6db2\u306b\u3064\u3051\u305f\u307e\u307e\u3001\u9178\u6027\u6db2 … \u7d9a\u304d\u3092\u8aad\u3080 STAP\u7d30\u80de\uff14\u3064\u306e\u30d7\u30ed\u30c8\u30b3\u30eb<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2,8],"tags":[],"class_list":["post-28247","post","type-post","status-publish","format-standard","hentry","category-education","category-fake"],"_links":{"self":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/28247","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28247"}],"version-history":[{"count":20,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/28247\/revisions"}],"predecessor-version":[{"id":28267,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/28247\/revisions\/28267"}],"wp:attachment":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28247"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28247"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28247"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}