{"id":27356,"date":"2025-11-25T16:35:21","date_gmt":"2025-11-25T07:35:21","guid":{"rendered":"https:\/\/nbsigh2.com\/?p=27356"},"modified":"2025-12-13T16:02:09","modified_gmt":"2025-12-13T07:02:09","slug":"%e3%82%a4%e3%83%b3%e3%83%97%e3%83%aa%e3%83%b3%e3%83%88%e3%81%a8%e3%82%ad%e3%83%a1%e3%83%a9%e8%83%bd","status":"publish","type":"post","link":"https:\/\/nbsigh2.com\/?p=27356","title":{"rendered":"\u30a4\u30f3\u30d7\u30ea\u30f3\u30c8\u3068\u30ad\u30e1\u30e9\u80fd"},"content":{"rendered":"

\u30a4\u30f3\u30d7\u30ea\u30f3\u30c8\u3068\u30ad\u30e1\u30e9\u80fd\u306b\u3064\u3044\u3066\u306e\u5b66\u3068\u307f\u5b50\u306e\u767a\u8a00\u3002<\/p>\n

\u305d\u3082\u305d\u3082\u306f2025\u5e7411\u670813\u65e5 07:15\u306b\u5f53\u65b9\u304cChatGPT\u306b\u8cea\u554f\u3057\u305f<\/a>\u3053\u3068\u306b\u306f\u3058\u307e\u308a\u307e\u3059\u3002\u5b66\u3068\u307f\u5b50\u304c\u9178\u6d74\u7d30\u80de\u3068\u63a5\u89e6\u3057\u305fES\u7d30\u80de\u306f\u3059\u3050\u306b\u3067\u3082\u30ad\u30e1\u30e9\u80fd\u3092\u5931\u3046\u3068\u3044\u3046\u6839\u62e0\u306e\u306a\u3044\u4e3b\u5f35\u3092\u3057\u305f\u305f\u3081\u3001\u5f53\u65b9\u304cChatGPT\u306b\u300cES\u7d30\u80de\u306b\u6700\u9069\u5316\u3055\u308c\u305f\u57f9\u990a\u74b0\u5883\u3067\u9178\u306b\u66b4\u9732\u3055\u308c\u305f\u7d30\u80de\u304c\u57f9\u990a\u3055\u308c\u3066\u3044\u308b\u57f9\u990a\u76bf\u306bES\u7d30\u80de\u304c\u6df7\u5165\u3057\u305f\u3068\u304d\u3001ES\u7d30\u80de\u306e\u30ad\u30e1\u30e9\u80fd\u304c\u76f4\u3061\u306b\u5931\u306a\u308f\u308c\u308b\u3068\u3044\u3046\u6839\u62e0\u306f\u3042\u308a\u307e\u3059\u304b\uff1f\u300d<\/span>\u3068\u5f53\u65b9\u3089\u306b\u3068\u3063\u3066\u306f\u3059\u3067\u306b\u7d50\u8ad6\u304c\u51fa\u3066\u3044\u308b\u8cea\u554f\u3092\u3057\u305f\u308f\u3051\u3067\u3059\u306d\u3002ChatGPT\u306f\u52ff\u8ad6\u3001\u6839\u62e0\u306f\u898b\u5f53\u305f\u3089\u306a\u3044\u3068\u5426\u5b9a\u3057\u305f\u308f\u3051\u3067\u3059\u304c\u3001\u305d\u306e\u969b\u3001 https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC6451320\/?utm_source=chatgpt.com<\/a> \u201cDefined conditions for propagation and manipulation of mouse embryonic stem cells\u3000Mulas …Austin Smith Development. 2019 Mar 26;146(6)\u201d\u3068\u3044\u3046\u8ad6\u6587\u304c\u5f15\u7528\u3055\u308c\u3066\u3044\u305f\u308f\u3051\u3067\u3059\u3002<\/p>\n

\u3053\u308c\u3092\u773a\u3081\u306b\u884c\u3063\u305f\u5b66\u3068\u307f\u5b50\u306fIntroduction\u306b\u300cFemale ES cells are vulnerable to severe hypomethylation (Choi et al., 2017a) and loss of methylation imprints in either 2i or serum, which can reduce their chimaera contribution (Yagi et al., 2017). \u96cc\u6027ES\u7d30\u80de\u306f\u30012i\u57f9\u990a\u6db2\u307e\u305f\u306f\u8840\u6e05\u306e\u3044\u305a\u308c\u306b\u304a\u3044\u3066\u3082\u91cd\u5ea6\u306e\u4f4e\u30e1\u30c1\u30eb\u5316\uff08Choi et al., 2017a\uff09\u304a\u3088\u3073\u30e1\u30c1\u30eb\u5316\u30a4\u30f3\u30d7\u30ea\u30f3\u30c8\u306e\u55aa\u5931\u3092\u53d7\u3051\u3084\u3059\u304f\u3001\u3053\u308c\u306b\u3088\u308a\u30ad\u30e1\u30e9\u5f62\u6210\u3078\u306e\u5bc4\u4e0e\u304c\u6e1b\u5c11\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\uff08Yagi et al., 2017\uff09\uff08DeepL\u8a33\uff09\u300d\u3068\u3044\u3046\u306e\u3092\u898b\u3064\u3051\u305f\u306e\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u3053\u306e\u8a18\u8ff0\u306b\u7279\u306b\u89e6\u308c\u308b\u306a\u304f\u3001\u3053\u306e\u300cHere, we provide detailed methodologies for derivation, propagation, genetic modification and primary differentiation of ES cells in 2i or 2i+LIF media without serum or undefined serum substitutes.\u3053\u3053\u3067\u306f\u3001\u8840\u6e05\u307e\u305f\u306f\u672a\u5b9a\u7fa9\u8840\u6e05\u4ee3\u66ff\u7269\u3092\u542b\u307e\u306a\u30442i\u307e\u305f\u306f2i+LIF\u57f9\u5730\u306b\u304a\u3051\u308bES\u7d30\u80de\u306e\u8a98\u5c0e\u3001\u7d99\u4ee3\u57f9\u990a\u3001\u907a\u4f1d\u5b50\u6539\u5909\u3001\u304a\u3088\u3073\u4e00\u6b21\u5206\u5316\u306b\u95a2\u3059\u308b\u8a73\u7d30\u306a\u65b9\u6cd5\u8ad6\u3092\u63d0\u4f9b\u3059\u308b\u3002\uff08DeepL\u8a33\uff09\u300d\u3068\u3044\u3046\u8ad6\u6587\u3092\u300cES\u306b\u3059\u308b\u6642\u306b\u304a\u304d\u308b\u30a4\u30f3\u30d7\u30ea\u30f3\u30c6\u30f3\u30b0\u9818\u57df\u306e\u30e1\u30c1\u30eb\u5316\u72b6\u614b\u3078\u306e\u30ea\u30b9\u30af\u306a\u3069\u3092\u8aac\u660e\u3057\u3001\u304b\u3064\u3001ES\u3092\u6271\u3046\u6642\u306e\u69d8\u3005\u306a\u624b\u9593\u3072\u307e\u304b\u304b\u308b\u624b\u6280\u306a\u3069\u3092\u793a\u3057\u305f\u8ad6\u6587\u3067\u3059\u3002\u300d<\/span><\/a>\u3064\u307e\u308aES\u7d30\u80de\u306e\u57f9\u990a\u306e\u96e3\u3057\u3055\u3092\u5531\u3048\u305f\u8ad6\u6587\u3068\u3001\u8aa4\u3063\u3066\u3068\u3089\u3048\u305f\u308f\u3051\u3067\u3059\u3002<\/p>\n

\u3000\u3061\u306a\u307f\u306b\u5f15\u7528\u3055\u308c\u305f\uff08Yagi et al., 2017\uff09\u3068\u306fDerivation of ground-state female ES cells maintaining gamete-derived DNA methylation\u3000Nature volume 548, pages 224\u2013227 (2017)<\/a>\u3068\u3044\u3046\u8ad6\u6587\u3067\u3001\u3053\u306e\u8ad6\u6587\u3067\u30ad\u30e1\u30e9\u306b\u3064\u3044\u3066\u89e6\u308c\u3066\u3044\u308b\u3068\u3053\u308d\u3092\u63a2\u3059\u3068
\n####\u8a33\u306f\u3044\u305a\u308c\u3082DeepL###
\nWe observed a further gain of de novo DNA methylation in 2i\/L-MEFs (Extended Data Fig. 3g), suggesting that female 2i\/L ES cells efficiently contribute to chimaeric embryos as they undergo de novo methylation during development. 2i\/L-MEFs\u306b\u304a\u3044\u3066\u65b0\u898fDNA\u30e1\u30c1\u30eb\u5316\u306e\u3055\u3089\u306a\u308b\u5897\u52a0\u3092\u89b3\u5bdf\u3057\u305f\uff08\u62e1\u5f35\u30c7\u30fc\u30bf\u56f33g\uff09\u3002\u3053\u308c\u306f\u3001\u96cc\u60272i\/L ES\u7d30\u80de\u304c\u80da\u767a\u751f\u4e2d\u306b\u65b0\u898f\u30e1\u30c1\u30eb\u5316\u3092\u53d7\u3051\u308b\u904e\u7a0b\u3067\u3001\u30ad\u30e1\u30e9\u80da\u3078\u306e\u52b9\u7387\u7684\u306a\u5bc4\u4e0e\u3092\u793a\u5506\u3057\u3066\u3044\u308b\u3002<\/p>\n

As reported previously2, we found that 2i\/L ES cells are consistently able to form chimaeric embryos that exhibit a high contribution of ES-cell-derived cells (Extended Data Fig. 6a). \u4ee5\u524d\u5831\u544a\u3057\u305f\u901a\u308a2\u3001\u6211\u3005\u306f2i\/L ES\u7d30\u80de\u304c\u4e00\u8cab\u3057\u3066ES\u7d30\u80de\u7531\u6765\u7d30\u80de\u306e\u9ad8\u3044\u5bc4\u4e0e\u7387\u3092\u793a\u3059\u30ad\u30e1\u30e9\u80da\u3092\u5f62\u6210\u3067\u304d\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u305f\uff08\u62e1\u5f35\u30c7\u30fc\u30bf\u56f36a\uff09\u3002<\/p>\n

We also compared DNA methylation and gene expressions of 2i\/L-MEFs derived from all-ES-cell embryos (4n 2i\/L-MEFs; E14.5) with those from chimaeric embryos (2n 2i\/L-MEFs; E14.5). Although no detectable difference in DNA methylations was observed between 4n 2i\/L-MEFs and 2n 2i\/L-MEFs (Extended Data Fig. 7i), many imprinted genes, as well as other genes, were aberrantly expressed in 4n 2i\/L-MEFs, whereas the aberration was less obvious in 2n 2i\/L-MEFs, presumably owing to non-cell-autonomous effects (Extended Data Fig. 7j)\u3000\u307e\u305f\u3001\u5168ES\u7d30\u80de\u80da\u7531\u6765\u306e2i\/L-MEFs\uff084n 2i\/L-MEFs; E14.5\uff09\u3068\u30ad\u30e1\u30e9\u80da\u7531\u6765\u306e2i\/L-MEFs\uff082n 2i\/L-MEFs; E14.5\uff09\u306eDNA\u30e1\u30c1\u30eb\u5316\u304a\u3088\u3073\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u6bd4\u8f03\u3057\u305f\u30024n 2i\/L-MEFs \u3068 2n 2i\/L-MEFs \u3067\u306f DNA \u30e1\u30c1\u30eb\u5316\u306b\u691c\u51fa\u53ef\u80fd\u306a\u5dee\u7570\u306f\u8a8d\u3081\u3089\u308c\u306a\u304b\u3063\u305f\uff08\u62e1\u5f35\u30c7\u30fc\u30bf\u56f3 7i\uff09\u3002\u591a\u304f\u306e\u30a4\u30f3\u30d7\u30ea\u30f3\u30c8\u907a\u4f1d\u5b50\u304a\u3088\u3073\u305d\u306e\u4ed6\u306e\u907a\u4f1d\u5b50\u304c4n 2i\/L-MEFs\u3067\u7570\u5e38\u767a\u73fe\u3057\u3066\u3044\u305f\u306e\u306b\u5bfe\u3057\u30012n 2i\/L-MEFs\u3067\u306f\u305d\u306e\u7570\u5e38\u304c\u3088\u308a\u4e0d\u660e\u77ad\u3067\u3042\u3063\u305f\u3002\u3053\u308c\u306f\u304a\u305d\u3089\u304f\u975e\u7d30\u80de\u81ea\u5f8b\u7684\u52b9\u679c\u306b\u3088\u308b\u3082\u306e\u3067\u3042\u308b\uff08\u62e1\u5f35\u30c7\u30fc\u30bf\u56f37j\uff09\u3002.
\n###
\n\u3068\u3044\u3046\u3088\u3046\u306a\u3053\u3068\u304c\u66f8\u3044\u3066\u3042\u308a\u3001\u5f53\u65b9\u306b\u306f\u3061\u3068\u96e3\u3057\u3044\u8ad6\u6587\u3067\u307e\u3061\u304c\u3048\u3066\u3044\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u304c\u300c\u30ad\u30e1\u30e9\u5f62\u6210\u3078\u306e\u5bc4\u4e0e\u304c\u6e1b\u5c11\u3059\u308b\u53ef\u80fd\u6027\u300d\u306a\u3069\u306f\u66f8\u3044\u3066\u306a\u304f\u3001\u9006\u306e\u3088\u3046\u306b\u601d\u3048\u307e\u3059\u3002\u3064\u307e\u308a\u30e1\u30b9\u306e\u7d30\u80de\u306e\u307b\u3046\u304c\u30a4\u30f3\u30d7\u30ea\u30f3\u30c8\u304c\u7834\u7dbb\u3057\uff08\u30e1\u30c1\u30eb\u5316\u304c\u306f\u305a\u308c\uff09\u3084\u3059\u304f\u3001\u30ad\u30e1\u30e9\u306b\u306a\u308a\u3084\u3059\u3044\u3068\u3044\u3046\u3088\u3046\u306b\u8aad\u3081\u305f\u306e\u3067\u3059\u304c\u9593\u9055\u3044\u3067\u3059\u304b\u306d\uff1f
\n\uff1e\u30a4\u30f3\u30d7\u30ea\u30f3\u30c8\u306b\u8a73\u3057\u3044\u5b66\u3068\u307f\u5b50
\nYagi\u7b49\u306e\u8ad6\u6587\u3092\u8aad\u3093\u3067\u89e3\u8aac\u3057\u3066\u3061\u3087\u3046\u3060\u3044\u3002\u5b6b\u5f15\u304d\u3067\u8b70\u8ad6\u3059\u308b\u306e\u306f\u3088\u308d\u3057\u304f\u306a\u3044\u306e\u306f\u308f\u304b\u308b\u3088\u306d\uff1f<\/p>\n

\u305d\u3057\u3066\u3001\u5b66\u3068\u307f\u5b50\u306f\u3053\u306eMulas\u7b49\u306e\u8ad6\u6587\u3092\u3001\u300cSTAP\u8ad6\u6587\u306eES\u306d\u3064\u9020\u306b\u5b9f\u884c\u72b6\u6cc1\u3092\u60f3\u50cf\u3059\u308b\u3068\u3001\u305d\u306e\u306d\u3064\u9020\u3092\u5b9f\u884c\u3055\u305b\u308b\u305f\u3081\u306b\u306f\u3001\u72af\u4eba\u306f\u3001\u7570\u306a\u308b\u5b9f\u9a13\u306e\u3042\u3061\u3053\u3061\u306e\u5834\u9762\u3067\u3001ES\u3092\u5165\u308c\u305f\u308a\u3001\u3059\u308a\u66ff\u3048\u305f\u308a\u3059\u308b\u884c\u70ba\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002\u30d9\u30c6\u30e9\u30f3\u7814\u7a76\u8005\u3092\u9a19\u3059\u305f\u3081\u306b\u306f\u3001\u3042\u3061\u3053\u3061\u306b\u4fee\u6b63\u304c\u5fc5\u8981\u3067\u3059\u3002
\n\u3057\u304b\u3057\u3001ES\u306f\u7dad\u6301\u3059\u308b\u3060\u3051\u3067\u3082\u5927\u5909\u3067\u3059\u3002\u305d\u3046\u3057\u305f\u51fa\u3057\u5165\u308c\u306e\u624b\u6280\u306b\u8010\u3048\u3089\u308c\u308b\u7d30\u80de\u3068\u306f\u601d\u3048\u305a\u3001ES\u306d\u3064\u9020\u8aac\u305d\u306e\u3082\u306e\u306b\u975e\u73fe\u5b9f\u6027\u304c\u3042\u308a\u307e\u3059\u3002
\n\u305d\u3053\u3067\u3001\u3053\u306e\u8ad6\u6587\u3092\u3001ES\u306d\u3064\u9020\u8aac\u3078\u306e\u4e00\u3064\u306e\u53cd\u8ad6\u3068\u3057\u3066\u4f7f\u3048\u307e\u305b\u3093\u304b\uff1f\u300d<\/span><\/a>\u3068ChatGPT\u306b\u8a98\u5c0e\u8cea\u554f\u3092\u3057\u305f\u306e\u3067\u3059\u3002\u3064\u307e\u308a\u3001ES\u7d30\u80de\u306e\u7dad\u6301\u306f\u5927\u5909\u3060\u304b\u3089\u3001ES\u7d30\u80de\u3092\u6df7\u5165\u3055\u305b\u308b\u305f\u3081\u306b\u306f\u8272\u3005\u64cd\u4f5c\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u306e\u3067\u3001ES\u7d30\u80de\u3092\u6df7\u5165\u3055\u305b\u308b\u3053\u3068\u306b\u3088\u308b\u30c7\u30fc\u30bf\u306e\u634f\u9020\u306f\u3067\u304d\u306a\u3044\u306e\u3067\u306f\u306a\u3044\u304b\uff1f\u3068ChatGPT\u306b\u805e\u3044\u305f\u308f\u3051\u3067\u3059\u3002<\/p>\n

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