{"id":25598,"date":"2024-08-12T07:40:39","date_gmt":"2024-08-11T22:40:39","guid":{"rendered":"https:\/\/nbsigh2.com\/?p=25598"},"modified":"2024-08-26T07:58:46","modified_gmt":"2024-08-25T22:58:46","slug":"%e5%b0%8f%e4%bf%9d%e6%96%b9%e3%83%91%e3%83%bc%e3%83%88%e3%81%af%e9%81%94%e6%88%90%e3%81%95%e3%82%8c%e3%81%9f%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/nbsigh2.com\/?p=25598","title":{"rendered":"\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u306f\u9054\u6210\u3055\u308c\u305f\uff1f"},"content":{"rendered":"

\u5b66\u3068\u307f\u5b50\u304c11\u65e5\uff08\u65e5\uff09\u5348\u5f8c\u65e9\u304f\u3001\u65b0\u3057\u3044\u8a18\u4e8b\u7406\u7814\u3092\u7ba1\u7406\u3059\u308b\u653f\u5e9c\u306f\u3001STAP\u4e8b\u4ef6\u306f\u3001\u5c0f\u4fdd\u65b9\u6c0f\u306b\u5168\u8cac\u4efb\u304c\u3042\u308b\u3068\u3044\u3046\u5f62\u3067\u53ce\u675f\u3055\u305b\u307e\u3057\u305f\u3002 \u3067\u3059\u304b\u3089\u3001\u3044\u308d\u3044\u308d\u306a\u77db\u76fe\u304c\u3042\u308b\u306e\u3067\u3059\u3002<\/a>\u3092\u30a2\u30c3\u30d7\u3057\u307e\u3057\u305f\u3002\u30bf\u30a4\u30c8\u30eb\u304c\u672c\u6587\u3092\u53cd\u6620\u3057\u3066\u3044\u306a\u3044\u306e\u306f\u3044\u3064\u3082\u306e\u3053\u3068\u3067\u3059\u3002URL\u304c81\u304b\u308984\u306b\u98db\u3093\u3067\u3044\u308b\u306e\u3067\u3001\u3053\u306e\u9593\u3044\u5e7e\u3064\u304b\u306e\u8a18\u4e8b\u3092\u66f8\u3044\u3066\u6d88\u3057\u305f\u306e\u3092\u304f\u308a\u304b\u3048\u3057\u305f\u306e\u3067\u3057\u3087\u3046\u3002\u3053\u306e\u8a18\u4e8b\u306b\u3064\u3044\u3066\u4ee5\u4e0b\u306b\u5b66\u3068\u307f\u5b50\u306b\u79d1\u5b66\u7684\u8ad6\u7406\u3092\u69cb\u7bc9\u3059\u308b\u80fd\u529b\u304c\u306a\u3044\u3053\u3068\u3092\u793a\u3057\u3066\u307f\u307e\u3057\u3087\u3002<\/p>\n

\u7406\u7814\u304c\u767a\u8868\u3057\u305f\u6700\u521d\u306e\u30d7\u30ec\u30b9\u30ea\u30ea\u30fc\u30b9<\/a>\u3067\u306f
\n\u300c\u5171\u540c\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306f\u3001\u3053\u306e\u521d\u671f\u5316\u73fe\u8c61\u3092\u523a\u6fc0\u60f9\u8d77\u6027\u591a\u80fd\u6027\u7372\u5f97\uff08STAP\uff09\u3001\u521d\u671f\u5316\u3055\u308c\u305f\u7d30\u80de\u3092STAP\u7d30\u80de<\/span>\u3068\u540d\u4ed8\u3051\u307e\u3057\u305f\u3002\u300d\u3068\u3042\u308a\u307e\u3059\u3002\u3064\u307e\u308a\u521d\u671f\u5316\u3055\u308c\u305f\u7d30\u80de\u304cSTAP\u7d30\u80de\u3067\u3042\u308a\u3001Oct3\/4\u306e\u767a\u73fe\u306f\u305d\u306e\u6307\u6a19\u3067\u3059\u304c\u30a4\u30b3\u30fc\u30eb\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u300cSTAP\u7d30\u80de\u306f\u7d30\u80de\u5206\u88c2\u3092\u3057\u3066\u5897\u6b96\u3059\u308b\u3053\u3068\u304c\u307b\u3068\u3093\u3069\u8d77\u304d\u306a\u3044\u7d30\u80de\u300d\u3060\u304c\u300c\u526f\u814e\u2f6a\u8cea\u523a\u6fc0\u30db\u30eb\u30e2\u30f3\u3092\u542b\u3080\u591a\u80fd\u6027\u7d30\u80de\u2f64\u306e\u7279\u6b8a\u306a\u57f9\u990a\u6db2\u3092\u2f64\u3044\u308b\u3053\u3068\u3067STAP\u7d30\u80de\u306e\u5897\u6b96\u3092\u4fc3\u3057\u3001STAP\u7d30\u80de\u304b\u3089ES\u7d30\u80de\u3068\u540c\u69d8\u306e\u2fbc\u3044\u5897\u6b96\u6027\uff08\u2f83\u2f30\u8907\u88fd\u80fd\uff09\u3092\u6709\u3059\u308b\u7d30\u80de\u682a\uff08STAP\u5e79\u7d30\u80de\uff09\u3092\u5f97\u308b\u2f45\u6cd5\u3082\u78ba\u2f74\u3057\u307e\u3057\u305f\u300d\u3068\u3042\u308a\u307e\u3059\u3002\u64a4\u56de\u3055\u308c\u305f\u8ad6\u6587\u3067\u3059\u304b\u3089\u3053\u308c\u3089\u306e\u7d30\u80de\u306b\u3064\u3044\u3066\u8b70\u8ad6\u3059\u308b\u306e\u306f\u610f\u5473\u306e\u306a\u3044\u3053\u3068\u3067\u3059\u304c\u3001\u305d\u308c\u3067\u3082\u30aa\u30ea\u30b8\u30ca\u30eb\u306e\u5b9a\u7fa9\u3092\u3057\u3063\u304b\u308a\u8e0f\u307e\u3048\u306a\u3044\u3068\u3001STAP\u7d30\u80de\u306e\u6709\u7121\u3001\u5c0f\u4fdd\u65b9\u6c0f\u304c\u521d\u671f\u5316\u3055\u308c\u305f\u7d30\u80de\u3092\u82e5\u5c71\u6c0f\u306b\u624b\u6e21\u3057\u305f\u306e\u304b\u7b49\u306e\u8b70\u8ad6\u304c\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n

\u5b66\u3068\u307f\u5b50\u3092\u59cb\u3081\u3068\u3059\u308b\u79d1\u5b66\u7684\u7d20\u990a\u306e\u306a\u3044\u64c1\u8b77\u306e\u9023\u4e2d\u306f\u3001STAP\u7d30\u80de\u30fbSTAP\u5e79\u7d30\u80de\u3092\u4f5c\u6210\u3059\u308b\u624b\u9806\u3067\u3001STAP\u7d30\u80de\u3092\u4f5c\u6210\u3059\u308b\u306e\u304c\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u3001\u30ad\u30e1\u30e9\u3084STAP\u5e79\u7d30\u80de\u3092\u4f5c\u6210\u3059\u308b\u306e\u304c\u82e5\u5c71\u30d1\u30fc\u30c8\u3068\u3057\u3066\u5206\u3051\u3001\u7d50\u679c\u3068\u3057\u3066ES\u7d30\u80de\u304c\u6df7\u5165\u3057\u3066\u3044\u305f\u3068\u3044\u3046\u4e8b\u5b9f\u3092\u8a8d\u3081\u3001\u305d\u306e\u6df7\u5165\u306f\u3069\u3061\u3089\u306e\u62c5\u5f53\u90e8\u5206\u3067\u8d77\u3053\u3063\u305f\u306e\u304b\u3092\u554f\u984c\u306b\u3057\u3066\u3044\u307e\u3059\u3002\u76f8\u6fa4\u6c0f\u4e3b\u5c0e\u306e\u5c0f\u4fdd\u65b9\u6c0f\u53c2\u52a0\u306e\u691c\u8a3c\u5b9f\u9a13\u306e\u7d50\u679c\u3084\u3001\u3053\u306e\u5b66\u3068\u307f\u5b50\u306e\u8a18\u4e8b\u306e\u3088\u3046\u306b\u4e39\u7fbd\u6c0f\u306e\u30ec\u30dd\u30fc\u30c8\u3092\u8aad\u307f\u3001\u64c1\u8b77\u306e\u8a00\u3046\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u306f\u6210\u529f\u3057\u305f\u3068\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u3057\u304b\u3057\u3001\u3082\u3057\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u3001\u82e5\u5c71\u30d1\u30fc\u30c8\u3068\u5206\u3051\u308b\u306e\u306a\u3089\u3001\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u3068\u306fSTAP\u7d30\u80de\u306e\u4f5c\u6210\u3068STAP\u7d30\u80de\u3068STAP\u5e79\u7d30\u80de\u306e\u89e3\u6790\u3001\u30c6\u30e9\u30c8\u30fc\u30de\u4f5c\u6210\u3067\u3042\u308a\u82e5\u5c71\u30d1\u30fc\u30c8\u3068\u306f\u30ad\u30e1\u30e9\u3068STAP\u5e79\u7d30\u80de\u306e\u4f5c\u6210\u3068\u3059\u3079\u304d\u3067\u3059\u3002\u524d\u8005\u306b\u7814\u7a76\u4e0d\u6b63\u304c\u3042\u3063\u305f\u3068\u3044\u3046\u3053\u3068\u3092\u5ff5\u982d\u306b\u7f6e\u304f\u3079\u304d\u3067\u3001\u30cd\u30ba\u30df\u52d5\u753b<\/a>\u306b\u30b3\u30e1\u30f3\u30c8\u3057\u3066\u3044\u308b\u30a8\u30b3\u30c1\u30a7\u30f3\u30d0\u30fc\u5185\u306e\u7121\u6559\u990a\u306a\u9023\u4e2d\u53ca\u3073\u5b66\u3068\u307f\u5b50\u306f\u3053\u306e\u5c0f\u4fdd\u65b9\u6c0f\u306e\u7814\u7a76\u4e0d\u6b63\u306e\u4e8b\u5b9f\u3092\u7121\u8996\u3057\u3001\u8a18\u8005\u4f1a\u898b\u3068\u79c1\u5c0f\u8aac\u306e\u300c\u5370\u8c61\u64cd\u4f5c\u300d\u306b\u9a19\u3055\u308c\u3066\u5c0f\u4fdd\u65b9\u6c0f\u3092\u64c1\u8b77\u3057\u3066\u3044\u308b\u306e\u306b\u904e\u304e\u307e\u305b\u3093\u3002<\/p>\n

\u64c1\u8b77\u306e\u8a00\u3046\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u304c\u306f\u6210\u529f\u3057\u305f\u3068\u3044\u3046\u6839\u62e0\u306f\u3001Oct-GFP\u3092\u4ed5\u8fbc\u3093\u3060\u7d30\u80de\u3067\u3001\u81ea\u5bb6\u86cd\u5149\u3067\u306f\u306a\u3044\u7dd1\u306e\u86cd\u5149\u3092\u767a\u3057\u3066\u3044\u308b\u7d30\u80de\u304c\u306a\u3044\u308f\u3051\u3067\u306f\u306a\u3044\uff08Green fluorescent cell aggregates that exhibited no significant redfluorescence were rare.<\/span>\uff08\u76f8\u6fa4\u30ec\u30dd\u30fc\u30c8<\/a>\uff09\u3001\u809d\u7d30\u80de\u3092\u4f7f\u3063\u305f\u5b9f\u9a13\u3067Oct3\/4\u3092\u767a\u73fe\u3057\u3066\u3044\u308b\u7d30\u80de\u304c\u3042\u3063\u305f\uff08\u4e39\u7fbd\u30ec\u30dd\u30fc\u30c8<\/a>\uff09\u3068\u3044\u3046\u3053\u3068\u3060\u3051\u306e\u3088\u3046\u3067\u3059\u3002\u5c11\u306a\u3044\u3068\u306f\u8a00\u3048\u3042\u3063\u305f\u3068\u3044\u3046\u3053\u3068\u304c\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u306f\u6210\u529f\u3060\u3063\u305f\u3068\u3044\u3046\u306e\u304c\u64c1\u8b77\u306e\u4e3b\u5f35\u3067\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u64c1\u8b77\u306e\u8a00\u3046\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u3067\u7dd1\u306b\u5149\u3063\u305f\u3001Oct3\/4 \u306e\u767a\u73fe\u304c\u3042\u3063\u305f\u3068\u3044\u3046\u3053\u3068\u306fSTAP\u7d30\u80de\u304c\u3067\u304d\u305f\u3068\u3044\u3046\u3053\u3068\u306b\u306f\u306a\u308a\u307e\u305b\u3093\u3002\u4e0a\u8a18\u306e\u3088\u3046\u306bSTAP\u7d30\u80de\u306f\u521d\u671f\u5316\u3055\u308c\u305f\u7d30\u80de\u3060\u304b\u3089\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u7dd1\u306b\u5149\u3063\u305f\u3001Oct3\/4 \u306e\u767a\u73fe\u304c\u3042\u3063\u305f\u7d30\u80de\u304c\u521d\u671f\u5316\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u3092\u8a3c\u660e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u6b8b\u5ff5\u306a\u304c\u3089\u3053\u306e\uff12\u3064\u306e\u30ec\u30dd\u30fc\u30c8\u3067\u306f\u30ad\u30e1\u30e9\u3092\u4f5c\u308b\u3053\u3068\u306b\u3088\u308b\u521d\u671f\u5316\u306e\u8a3c\u660e\u306b\u5931\u6557\u3057\u307e\u3057\u305f\u3002\u3057\u305f\u304c\u3063\u3066\u64c1\u8b77\u306e\u8a00\u3046\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u306f\u6210\u529f\u3057\u305f\u3068\u3044\u3046\u6839\u62e0\u306f\u5168\u304f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n

\u30b9\u30c8\u30ec\u30b9\u306b\u7f6e\u304b\u308c\u305f\u7015\u6b7b\u72b6\u614b\u306e\u7d30\u80de\u304c\u7d71\u7387\u306e\u53d6\u308c\u305f\u53cd\u5fdc\u3068\u3057\u3066Oct3\/4\u3092\u767a\u73fe\u3057\u305f\u3068\u3044\u3046\u6839\u62e0\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u7d30\u80de\u306fOct3\/4\u3092\u767a\u73fe\u3057\u305f\u3067\u3057\u3087\u3046\u3051\u3069\u3001\u6b7b\u306c\u9593\u969b\u306e\u3067\u305f\u3089\u3081\u306a\u767a\u73fe\u304c\u3042\u3063\u305f\u306e\u3060\u3068\u8a00\u308f\u308c\u3066\u53cd\u8ad6\u3067\u304d\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u521d\u671f\u5316\u3055\u308c\u305f\u3053\u3068\u3092\u8a3c\u660e\u3059\u308c\u3070\u3044\u3044\u306e\u3067\u3059\u304c\u3067\u304d\u3066\u306a\u3044\u308f\u3051\u3067\u3059\u306d\u3002\u7e70\u308a\u8fd4\u3057\u307e\u3059\u304c\u64c1\u8b77\u66f0\u304f\u306e\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u304c\u521d\u671f\u5316\u3057\u305f\u7d30\u80de\u3092\u4f5c\u308b\u3068\u3044\u3046\u3053\u3068\u306a\u3089\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u306e\u518d\u73fe\u306f\u5931\u6557\u3057\u305f\u306e\u3067\u3059\u3002\u64c1\u8b77\u306e\u8a00\u3046\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u304c\u7dd1\u306b\u5149\u308b\u3042\u308b\u3044\u306fOct3\/4\u306e\u767a\u73fe\u304c\u3042\u308b\u51dd\u96c6\u584a\u306b\u306a\u3063\u305f\u3068\u3044\u3046\u3053\u3068\u306a\u3089\u6210\u529f\u3057\u305f\u5834\u5408\u304c\u3042\u308b\u3068\u3057\u3066\u3082\u3044\u3044\u3067\u3059\u3088\u3002\u3057\u304b\u3057\u305d\u306e\u3088\u3046\u306a\u300c\u5c0f\u4fdd\u65b9\u7d30\u80de\u300d\u306f\u767a\u751f\u5b66\u3084\u518d\u751f\u533b\u7642\u306b\u3069\u306e\u3088\u3046\u306a\u610f\u7fa9\u304c\u3042\u308b\u306e\u3067\u3057\u3087\uff1f<\/p>\n

\u306f\u3044\u3001\u5f53\u65b9\u306f\u300c\u5c0f\u4fdd\u65b9\u6c0f\u30d1\u30fc\u30c8\u306f\u9054\u6210\u3057\u3066\u3044\u305f\u300d<\/span>\u3068\u3044\u3046\u30cd\u30ba\u30df\u6c0f\u3084\u5b66\u3068\u307f\u5b50\u306e\u8003\u3048\u3092\u5426\u5b9a\u3057\u307e\u3057\u305f\u3002\u79d1\u5b66\u7684\u306a\u53cd\u8ad6\u3092\u3001\u6839\u62e0\u3092\u6dfb\u3048\u3066\u3069\u3046\u305e\u3002<\/p>\n

\u300c\u30cd\u30ba\u30df\u6c0f\u306f\u3001\u4e39\u7fbd\u8ad6\u6587\u3082\u3001\u5927\u4e8b\u306a\u3068\u3053\u308d\u3092\u62bc\u3055\u3048\u3066\u3044\u307e\u3059\u3002\u3059\u306a\u308f\u3061\u3001\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u3068\u3001\u82e5\u5c71\u30d1\u30fc\u30c8\u3092\u3057\u3063\u304b\u308a\u308f\u3051\u3066\u3001\u8ad6\u3058\u3066\u3044\u307e\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u4e0a\u8a18\u306e\u3088\u3046\u306b\u9593\u9055\u3044\u3067\u3059\u3002\u5206\u3051\u3066\u3082\u3044\u3044\u3067\u3059\u304c\u3001\u4e0d\u6b63\u884c\u70ba\u3092\u3057\u305f\u90e8\u5206\u3082\u5c0f\u4fdd\u65b9\u6c0f\u306e\u8cac\u4efb\u3067\u3059\u3002<\/p>\n

\u300c\uff08\u30cd\u30ba\u30df\u52d5\u753b\u306f\uff09\u518d\u73fe\u5b9f\u9a13\u3067\u306f\u3001\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u306f\u9054\u6210\u3057\u3066\u3044\u305f\u3068\u8aac\u660e\u3092\u3057\u3066\u3044\u307e\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u76f8\u6fa4\u6c0f\u306e\u691c\u8a3c\u5b9f\u9a13\u3084\u4e39\u7fbd\u6c0f\u306e\u30ec\u30dd\u30fc\u30c8\u306f\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\uff08\u521d\u671f\u5316\u3055\u308c\u305f\u7d30\u80de\u3092\u4f5c\u308b\uff09\u304c\u9054\u6210\u3067\u304d\u3066\u306a\u3044\u3068\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u30cd\u30ba\u30df\u6c0f\u306f\u7406\u89e3\u3067\u304d\u3066\u306a\u3044\u3068\u3044\u3046\u3053\u3068\u3067\u3059\u3002\u5b66\u3068\u307f\u5b50\u306f\u3057\u3063\u304b\u308a\u8aad\u3093\u3060\u306e\u3067\u3057\u3087\uff1f\u30cd\u30ba\u30df\u6c0f\u3092\u652f\u6301\u3059\u308b\u306e\uff1f<\/p>\n

\u300c\u7406\u7814\u3092\u7ba1\u7406\u3059\u308b\u653f\u5e9c\u306f\u3001STAP\u4e8b\u4ef6\u306f\u3001\u5c0f\u4fdd\u65b9\u6c0f\u306b\u5168\u8cac\u4efb\u304c\u3042\u308b\u3068\u3044\u3046\u5f62\u3067\u53ce\u675f\u3055\u305b\u307e\u3057\u305f\u3002\u3067\u3059\u304b\u3089\u3001\u3044\u308d\u3044\u308d\u306a\u77db\u76fe\u304c\u3042\u308b\u306e\u3067\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u3069\u3093\u306a\u77db\u76fe\u304c\u3042\u308b\u306e\u304b\u3092\u66f8\u304b\u305a\u3001\u3042\u308b\u3042\u308b\u3068\u3044\u3046\u306e\u306f\u5b66\u3068\u307f\u5b50\u306e\u3044\u3064\u3082\u306e\u5984\u60f3\u8868\u73fe\u3067\u3059\u3002\u77db\u76fe\u3068\u306f\u306a\u3093\u3067\u3057\u3087\u3046\u304b\u306d\uff1f<\/p>\n

\u300c\u6842\u6c0f\u306e\u8aac\u660e\u3082\u3001\u7a81\u3064\u304f\u3068\u79d1\u5b66\u7684\u77db\u76fe\u304c\u51fa\u3066\u304f\u308b\u306e\u3067\u3059\u304c\u3001\u8a18\u8005\u306b\u3082\u7a81\u3063\u3064\u304f\u6c17\u304c\u306a\u304b\u3063\u305f\u306e\u3067\u3059\u306d\u300d<\/span>\u3000\u2190\u3000\u3069\u3053\u306b\u77db\u76fe\u304c\u3042\u308b\u306e\u304b\u6307\u6458\u3057\u306a\u3044\u3067\u3001\u300c\u79d1\u5b66\u7684\u77db\u76fe\u304c\u51fa\u3066\u304f\u308b\u300d<\/span>\u3068\u3069\u3046\u3057\u3066\u3044\u3048\u308b\u306e\uff1f<\/p>\n

\u300c\u7406\u7814\u306e\u88c1\u5b9a\u306f\u3001\u5b9f\u9a13\u8005\u306e\u5f79\u5272\u5206\u62c5\u3068\u3044\u3046\u3082\u306e\u306b\u7126\u70b9\u3092\u5f53\u3066\u3066\u3044\u307e\u305b\u3093\u3002\u307b\u307c\u3001\u30d6\u30e9\u30c3\u30af\u30dc\u30c3\u30af\u30b9\u306b\u3057\u307e\u3057\u305f\u3002\u300d<\/span>\u3000\u2190\u3000\u8abf\u67fb\u59d4\u54e1\u4f1a\u306e\u5831\u544a\u3067\u306f\u300c\u30d6\u30e9\u30c3\u30af\u30dc\u30c3\u30af\u30b9\u300d<\/span>\u306b\u3057\u3066\u3044\u307e\u305b\u3093\u3002\u4e0a\u8a18\u306e\u3088\u3046\u306b\u82e5\u5c71\u6c0f\u62c5\u5f53\u306f\u30ad\u30e1\u30e9\u4f5c\u6210\u3068\u5e79\u7d30\u80de\u4f5c\u6210\u306e\u90e8\u5206\u3060\u3051\u3067\u3001\u307b\u304b\u306f\u3059\u3079\u3066\u5c0f\u4fdd\u65b9\u6c0f\u304c\u62c5\u5f53\u3057\u305f\u306e\u3067\u3059\u3002<\/p>\n

\u5b66\u3068\u307f\u5b50\u306f\u4e39\u7fbd\u6c0f\u8ad6\u6587\u306e\u300cThe efficiency of aggregate formation was clearly higher for ATP-treated cells than for HCl-treated or non-treated cells, especially in the case of liver cells.\u300d\u3092\u5f15\u7528\u3057\u3066\u51dd\u96c6\u584a\u304c\u3067\u304d\u305f\u3068\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u305d\u306e\u524d\u306e\u6587\u7ae0\uff1a\u300cHowever, although massive cell death was observed at two days after treatment, aggregate formation was rarely observed in any cell type (Fig. 1b). Occasional formation of aggregateswas also observed in the culture of non-treated cells, suggesting that low-pH treatment does not enhance the formation of cell aggregates.<\/span> \u3057\u304b\u3057\u306a\u304c\u3089\uff08\u5869\u9178\u306e\u5834\u5408\u306f\uff09\u51e6\u7406\u5f8c2\u65e5\u76ee\u306b\u5927\u91cf\u306e\u7d30\u80de\u6b7b\u304c\u89b3\u5bdf\u3055\u308c\u305f\u3082\u306e\u306e\u3001\u51dd\u96c6\u584a\u306e\u5f62\u6210\u306f\u3069\u306e\u7d30\u80de\u30bf\u30a4\u30d7\u3067\u3082\u307b\u3068\u3093\u3069\u89b3\u5bdf\u3055\u308c\u306a\u304b\u3063\u305f\uff08\u56f31b\uff09\u3002\u51dd\u96c6\u584a\u306e\u5f62\u6210\u306f\u3001\u975e\u51e6\u7406\u7d30\u80de\u306e\u57f9\u990a\u3067\u3082\u6642\u6298\u89b3\u5bdf\u3055\u308c\u305f\u3053\u3068\u304b\u3089\u3001\u4f4epH\u51e6\u7406\u306b\u3088\u3063\u3066\u51dd\u96c6\u584a\u306e\u5f62\u6210\u304c\u4fc3\u9032\u3055\u308c\u308b\u3053\u3068\u306f\u306a\u3044<\/span>\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u305f\u3002\u300d\u3092\u7121\u8996\u3059\u308b\u308f\u3051\u3067\u3059\u3002\u3067\u304d\u305f\u3067\u304d\u306a\u3044\u306e\u8b70\u8ad6\u306f\u3001\uff11\u30b1\u3067\u3082\u3067\u304d\u305f\u3089\u3067\u304d\u305f\u3068\u3059\u308b\u3068\u3057\u3066\u3082\u3044\u3044\u306e\u3067\u3059\u304c\u3001\u691c\u8a3c\u5b9f\u9a13\u3067\u3059\u304b\u3089\u30aa\u30ea\u30b8\u30ca\u30eb\u8ad6\u6587\u540c\u69d8\u304b\u304c\u554f\u984c\u3067\u7d30\u80de\u51dd\u96c6\u584a\u304c\u5f8c\u306e\u5b9f\u9a13\u306b\u4f7f\u3048\u308b\u307b\u3069\u306e\u91cf\u304c\u306a\u3051\u308c\u3070\u610f\u5473\u304c\u306a\u3044\u306e\u3067\u3059\u3002\u4e39\u7fbd\u6c0f\u306f\u5869\u9178\u3067\u306frarely observed\u3068\u8868\u73fe\u3057\u3066\u3044\u308b\u3053\u3068\u304b\u3089\u308f\u304b\u308b\u3088\u3046\u306b\u3001\u518d\u73fe\u3067\u304d\u306a\u304b\u3063\u305f\u306e\u3067\u3059\u3002\u3057\u304b\u3057ATP\u3092\u4f7f\u3063\u305f\u809d\u7d30\u80de\u306e\u5834\u5408\u306f\u51dd\u96c6\u584a\u304c\u3067\u304d\u305f\u3068\u3057\u3066\u3044\u308b\u306e\u3067\u3059\u3002\u3067\u3059\u304b\u3089\u3053\u308c\u4ee5\u964d\u809d\u7d30\u80de\u3092\u4f7f\u3063\u305f\u89e3\u6790\u3092\u3057\u3066\u3044\u308b\u306e\u3067\u3059\u306d\u3002\u3053\u306e\u4e39\u7fbd\u6c0f\u306e\u30ec\u30dd\u30fc\u30c8\u306f\u691c\u8a3c\u5b9f\u9a13\u306e\u3088\u3046\u306b\u898b\u3048\u307e\u3059\u304c\u3001\u809d\u7d30\u80de\u3092\u4f7f\u3063\u305f\u89e3\u6790\u3068\u3044\u3046\u306e\u306f\u691c\u8a3c\u5b9f\u9a13\u3092\u9038\u8131\u3057\u3066\u3044\u307e\u3059\u3002\u5171\u8457\u8005\u306a\u306e\u3067\u306a\u3093\u3068\u304bpositive\u306a\u7d50\u679c\u3092\u5f97\u305f\u3044\u3068\u3057\u305f\u306e\u304c\u3088\u304f\u308f\u304b\u308a\u307e\u3059\u306a\u3002<\/p>\n

\u300c\u809d\u81d3\u306e\u7d30\u80de\u3068\u3001\u813e\u81d3\u306e\u7d30\u80de\u306e\u5b9f\u9a13\u3092\u304d\u3061\u3093\u3068\u8aad\u307f\u5206\u3051\u308b\u3053\u3068\u3002\u300d<\/span>\u3000\u2190\u3000\u3060\u304b\u3089\u4f55\u6545\u4e39\u7fbd\u6c0f\u306f\u809d\u7d30\u80de\u3092\u4f7f\u3063\u305f\u306e\uff1f\u5b66\u3068\u307f\u5b50\u306e\u8003\u3048\u3092\u8a00\u3063\u3066\u307f\u3002<\/p>\n

\u300cES\u4e26\u307f\u306eOct3\/4\u767a\u73fe\u304c\u3042\u3063\u305f\u3068\u306e\u8a18\u8f09\u3067\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u3068\u3044\u3063\u3066\u4e39\u7fbd\u6c0f\u306e\u30ec\u30dd\u30fc\u30c8\u306eFig.3c \u3092\u5f15\u7528\u3057\u3066\u3044\u307e\u3059\u304c\u3000Fig3a \u3067\u306f\u5869\u9178\u3042\u308b\u3044\u306fATP\u51e6\u7406\uff17\u65e5\u5f8c\u306eQ-PCR\u89e3\u6790\u3067\u306f\u300cusing a total RNA sample prepared from all cells in the culture (Fig. 3a), indicating that extremely few or no cells expressing Oct3\/4 were present.\u5168\u4f53\u3067\u306f\u307b\u3068\u3093\u3069\u306a\u3044\u300d\u3068\u3042\u308a\u500b\u3005\u306e\u7d30\u80de\u584a\u306719%\uff085\/27\uff09\u767a\u73fe\u304c\u307f\u3089\u308c\u305f\u3001\u3064\u307e\u308aOct3\/4\u306e\u767a\u73fe\u306f\u975e\u5e38\u306b\u5c11\u306a\u3044\u3068\u3044\u3046\u8868\u73fe\u3067\u3059\u306d\u3002<\/p>\n

\u4e39\u7fbd\u6c0f\u30ec\u30dd\u30fc\u30c8\u306e\u300cAs a result, we have concluded that the STAP phenomenon as described in the original studies is not reproducible.\u300d\u3092\u5f15\u7528\u3057\u3066\u300c\u82e5\u5c71\u30d1\u30fc\u30c8\u306e\u8cac\u4efb\u304c\u91cd\u3044\u3053\u3068\u3092\u3001\u30cd\u30ba\u30df\u6c0f\u3082\u3001\u6307\u6458\u3057\u3066\u3044\u307e\u3059\u3002\u300d<\/span>\u3068\u5b66\u3068\u307f\u5b50\u304c\u66f8\u304f\u306e\u3067\u3059\u304c\u3001\u8ad6\u7406\u304c\u308f\u304b\u308a\u307e\u305b\u3093\u306a\u3002\u521d\u671f\u5316\u3055\u308c\u305f\u7d30\u80de\u3092\u5c0f\u4fdd\u65b9\u6c0f\u304c\u4f5c\u308c\u306a\u304b\u3063\u305f\u3068\u3044\u3046\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3001\u3064\u307e\u308a\u307e\u3068\u3082\u306a\u6750\u6599\u3082\u306a\u3044\u306e\u306b\u30ad\u30e1\u30e9\u4f5c\u6210\u3092\u62c5\u5f53\u3057\u305f\u82e5\u5c71\u6c0f\u306e\u8cac\u4efb\u304c\u91cd\u3044\u3068\u3044\u3046\u3046\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n

\u300c\u30ad\u30e1\u30e9\u3092\u76ee\u7684\u3068\u3057\u305f\u518d\u73fe\u5b9f\u9a13\u306f\u6210\u529f\u3057\u306a\u304b\u3063\u305f\u3068\u3057\u3066\u3044\u308b\u3060\u3051\u3067\u3001\u5c0f\u4fdd\u65b9\u30d1\u30fc\u30c8\u306f\u8a3c\u660e\u3057\u3066\u3044\u307e\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u3061\u304c\u3044\u307e\u3059\u3002\u5c0f\u4fdd\u65b9\u6c0f\u304c\u521d\u671f\u5316\u3055\u308c\u305f\u7d30\u80de\uff1dSTAP\u7d30\u80de\u3092\u4f5c\u308c\u306a\u304b\u3063\u305f\u306e\u3067\u3059\u3002<\/p>\n

\u9178\u6027\u6db2\u306b\u6d78\u3059\u65b9\u6cd5\u306b\u3064\u3044\u3066\u3001\u3053\u306e\u8a18\u4e8b\u3067\u5b66\u3068\u307f\u5b50\u306f\u5f53\u65b9\u304c\u8ad6\u6587\u3092\u8aad\u3081\u3066\u306a\u3044\u3068\u6279\u5224\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u5f53\u65b9\u306f\u3059\u3067\u306b\u5404\u7a2e\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u6bd4\u8f03\u3057<\/a>\u3001\u300c\u5b66\u3068\u307f\u5b50\u306f\u7d30\u80de\u3092\u57f9\u990a\u3057\u3066\u3044\u308b\u6db2\u306bATP\u3042\u308b\u3044\u306f\u5869\u9178\u3092\u52a0\u3048\u308b\u306e\u3068\u3001\u96c6\u3081\u305f\u7d30\u80de\u306b\u3042\u3089\u304b\u3058\u3081\u9178\u6027\u306b\u3057\u305f\u57f9\u990a\u6db2\u6db2\u3092\u52a0\u3048\u308b\u306e\u3068\u306b\u3069\u3093\u306a\u5dee\u304c\u3042\u308b\u3068\u3044\u3046\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u300d<\/span>\u3068\u8cea\u554f\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u7b54\u3048\u304c\u300c\u9178\u6d74\u306e\u65b9\u6cd5\u306e\u9055\u3044\u304c\u3001\u7d30\u80de\u306b\u4e0e\u3048\u308b\u5f71\u97ff\u306e\u9055\u3044\u306b\u3064\u3044\u3066\u8a9e\u308c\u308b\u4eba\u306f\u3044\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u300d<\/span>\u306a\u3093\u3060\u305d\u3046\u3067\u3059\u3002\u99ac\u9e7f\u305d\u306e\u3082\u306e\u3067\u3059\u306d\u3002\u5b66\u3068\u307f\u5b50\u304c\u9060\u5fc3\u3057\u3066\u96c6\u3081\u305f\u7d30\u80de\u306b\u57f9\u990a\u6db2\u3092\u52a0\u3048\u9178\u3092\u305f\u3089\u3059\u306e\u3068\u9178\u6027\u306b\u3057\u305f\u57f9\u990a\u6db2\u3092\u6ce8\u3050\u306e\u3068\u306b\u9055\u3044\u304c\u3042\u308b\u3068\u3044\u3063\u305f\u306e\u3060\u304b\u3089\u3001\u3069\u3093\u306a\u9055\u3044\u304c\u3042\u308b\u306e\u304b\u3068\u5b66\u3068\u307f\u5b50\u306b\u805e\u3044\u3066\u3044\u308b\u306e\u3060\u304b\u3089\u3001\u8003\u3048\u3092\u7b54\u3048\u305f\u3089\u3044\u3044\u3067\u3057\u3087\u3002\u8ab0\u306b\u3082\u308f\u304b\u3089\u306a\u3044\u306e\u306a\u3089\u65b9\u6cd5\u306b\u3088\u308b\u9055\u3044\u304c\u306a\u3044\u3068\u3044\u3046\u306e\u3092\u5426\u5b9a\u3067\u304d\u306a\u3044\u3060\u308d\u3002\u5b66\u3068\u307f\u5b50\u306e\u8003\u3048\u3092\u8a00\u3063\u3066\u307f\u306a\u3002<\/p>\n

\u300c\u305f\u3081\u606f\u3055\u3093\u306b\u3082\u3001\u5b66\u3068\u307f\u5b50\u306b\u306f\u7b54\u3048\u304c\u7121\u3044\u306e\u306f\u660e\u3089\u304b\u3067\u3059\u3002\u3044\u3065\u308c\u306b\u3057\u308d\u3001\u9178\u6d74\u624b\u6280\u304c\u3001\u9055\u3046\u65b9\u6cd5\u3067\u3042\u3063\u305f\u3089\u3001\u8a71\u306b\u306a\u308a\u307e\u305b\u3093\u3002\u300d<\/span>\u3000\u2190\u3000\u3060\u3063\u305f\u3089\u65b9\u6cd5\u306e\u9055\u3044\u304c\u539f\u56e0\u3068\u3044\u3046\u6839\u62e0\u306f\u306a\u3044\u3068\u3044\u3046\u3053\u3068\u3060\u308d\u3002\u3053\u306e\u99ac\u9e7f\u306f\u79d1\u5b66\u7684\u8b70\u8ad6\u3092\u3057\u305f\u3053\u3068\u304c\u306a\u3044\u306e\u3067\u3059\u306a\u3002<\/p>\n

\u5b66\u3068\u307f\u5b50\u306e\u8868\u73fe\u300c\u5c0f\u4fdd\u65b9\u6c0f\u306e\u9178\u6027\u6db2\u306f\u3001\u9178\u6027\u6db2\u3092\u4f5c\u3063\u3066\u6f2c\u3051\u308b\u65b9\u6cd5\u3067\u306f\u306a\u3044\u300d\u300c\u4e39\u7fbd\u6c0f\u3084\u3001\u5c0f\u4fdd\u65b9\u6c0f\u306e\u9178\u6027\u6db2\u306f\u3001\u9178\u6027\u6db2\u3092\u4f5c\u3063\u3066\u6f2c\u3051\u308b\u65b9\u6cd5\u3067\u306f\u306a\u3044\u300d<\/span>\u3000\u306f\u65e5\u672c\u8a9e\u306b\u306a\u3063\u3066\u306a\u3044\u3068\u3044\u3046\u6279\u5224\u306b\u306a\u3093\u3068\u3082\u601d\u308f\u306a\u3044\u306e\u3067\u3059\u304b\u306d\uff1f\u300c\u9178\u6027\u6db2\u306f\u65b9\u6cd5\u3067\u306f\u306a\u3044\u300d<\/span>\u3068\u306f\u5c0f\u5b66\u751f\u3067\u3082\u66f8\u304b\u306a\u3044\u6587\u7ae0\u3060\u3068\u3044\u3046\u306e\u304c\u308f\u304b\u3089\u306a\u3044\u306e\uff1f<\/p>\n

\u300c\u3064\u307e\u308a\u3001\u4e16\u754c\u306e\u518d\u73fe\u5b9f\u9a13\u306f\u3001\u5c0f\u4fdd\u65b9\u6c0f\u306e\u65b9\u6cd5\u3068\u306f\u9055\u3063\u3066\u3044\u305f\u306e\u3067\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u300c\u5c0f\u4fdd\u65b9\u6c0f\u306e\u65b9\u6cd5\u300d<\/span>\u3000\u3092\u4f7f\u3063\u305f\u5c0f\u4fdd\u65b9\u6c0f\u81ea\u8eab\u306e\u518d\u73fe\u5b9f\u9a13\u3067\u3082\u518d\u73fe\u3067\u304d\u306a\u304b\u3063\u305f\u306e\u3067\u3059\u3002<\/p>\n

\u300c\u8ad6\u6587\u306b\u66f8\u3044\u3066\u3044\u306a\u3051\u308a\u3083\u3001\u518d\u73fe\u5b9f\u9a13\u3092\u3059\u308b\u7814\u7a76\u8005\u306f\u3001\u8457\u8005\u306e\u5c0f\u4fdd\u65b9\u6c0f\u306b\u805e\u304f\u3079\u304d\u3067\u3057\u3087\u3046\u3002\u300d<\/span>\u3000\u2190\u3000\u805e\u3044\u305f\u3068\u3053\u308d\u3067\u95a2\u4fc2\u8005\u304c\u8907\u6570\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u51fa\u3059\u306e\u3067\u3059\u304b\u3089\u3001\u805e\u3044\u3066\u3082\u3057\u3087\u3046\u304c\u306a\u3044\u3067\u3057\u3087\u3002\u3044\u305a\u308c\u3044\u3057\u308d\u3001\u3067\u304d\u306a\u3044\u306e\u3060\u304b\u3089\u306d\u3002<\/p>\n

\u300c\u3044\u3065\u308c\u306b\u3057\u308d\u3001\u30cd\u30ba\u30df\u6c0f\u306e\u52d5\u753b\u3068\u305d\u306e\u30b3\u30e1\u30f3\u30c8\u3092\u8aad\u3080\u9650\u308a\u3001STAP\u7d30\u80de\u64c1\u8b77\u8ad6\u3068\u3044\u3046\u306e\u306f\u3001\u5e83\u304f\u3001\u4e16\u9593\u306e\u652f\u6301\u304c\u3042\u308b\u3053\u3068\u304c\u3001\u305f\u3081\u606f\u30d6\u30ed\u30b0\u306b\u5206\u304b\u3063\u305f\u3067\u3057\u3087\u3046\u3002\u300d<\/span>\u3000\u2190\u3000\u5b66\u3068\u307f\u5b50\u3068\u540c\u69d8\u306b\u3001\u3053\u308c\u3060\u3051\u691c\u8a3c\u5b9f\u9a13\u3084\u8abf\u67fb\u306e\u7d50\u679c\u304c\u3042\u308b\u306e\u306b\u79d1\u5b66\u7684\u7d20\u990a\u306e\u306a\u3044\u7406\u89e3\u3067\u304d\u306a\u3044\u8f29\u304c\u3044\u3063\u3071\u3044\u3044\u308b\u3068\u3044\u3046\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u306a\u3002\u307f\u306a\u3055\u3093\u9a19\u3055\u308c\u305f\u3053\u3068\u306b\u6c17\u304c\u4ed8\u304b\u306a\u3044\u80fd\u5929\u6c17\u306a\u65b9\u3070\u304b\u308a\u306a\u3093\u3067\u3059\u306a\u3002<\/p>\n

\u300c\u5b66\u3068\u307f\u5b50\u3060\u3051\u304c\u8a00\u3063\u3066\u3044\u308b\u3068\u3044\u3046\u8a00\u3044\u65b9\u306f\u3001\u4eca\u5f8c\u306e\u305f\u3081\u606f\u30d6\u30ed\u30b0\u306f\u3067\u304d\u306a\u304f\u306a\u308a\u307e\u3057\u305f\u3002\u300d<\/span>\u3000\u2190\u3000\u4e8b\u6545\u3067ES\u7d30\u80de\u304c\u6df7\u5165\u3057\u305f\u3068\u3044\u3063\u3066\u3044\u308b\u306e\u306f\u5b66\u3068\u307f\u5b50\u3060\u3051\u3067\u3059\u3002\u3061\u304c\u3044\u307e\u3059\u304b\uff1f\u3060\u308c\u304b\u540c\u8abf\u3057\u3066\u3044\u308b\u65b9\u304c\u3044\u308b\u306e\uff1f<\/p>\n

\u300c\u305f\u3081\u606f\u3055\u3093\u3001\u3053\u3093\u306a\u3053\u3068\u3092\u8a00\u3063\u3066\u3044\u308b\u3068\u3001\u4e00\u822c\u4eba\u306b\u3082\u30d0\u30ab\u306b\u3055\u308c\u307e\u3059\u3088\u3002\u300d<\/span>
\n\u5b66\u3068\u307f\u5b50\u306e\u6307\u793a\u4ee3\u540d\u8a5e\u306f\u8ad6\u7406\u304c\u306a\u3044\u304b\u3089\u300c\u3053\u3093\u306a\u3053\u3068\u300d\u304c\u4f55\u3092\u6307\u3057\u3066\u3044\u308b\u306e\u304b\u308f\u304b\u3089\u306a\u3044\u306e\u3067\u3059\u304c\u3001\u305d\u306e\u524d\u306b\u5f15\u7528\u3057\u3066\u3044\u308b\u5f53\u65b9\u306e\u767a\u8a00\u306e\u3069\u308c\u304b\u306a\u308f\u3051\u3067\u3059\u306e\u3067\u30ea\u30b9\u30c8\u3059\u308b\u3068\uff1a
\n\u2460\u3069\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u5f93\u3063\u3066\u3082\u3001\u672c\u4eba\u3092\u542b\u3081\u8ab0\u3082\u518d\u73fe\u3067\u304d\u306a\u304b\u3063\u305f\u3000\u2190\u3000\u4e8b\u5b9f\u3060
\n\u2461\u6b8b\u3055\u308c\u305f\u30b5\u30f3\u30d7\u30eb\u306f\u8abf\u3079\u305f\u9650\u308a\u3059\u3079\u3066ES\u7d30\u80de\u7531\u6765\u3000\u2190\u3000\u4e8b\u5b9f\u3060
\n\u2462\u518d\u73fe\u3067\u304d\u306a\u304b\u3063\u305f\u539f\u56e0\u3092\u3001\u9178\u6027\u6db2\u306b\u6f2c\u3051\u308b\u65b9\u6cd5\u304c\u9055\u3046\u306a\u3069\u3068\u63a2\u308b\u306e\u306f\u7121\u610f\u5473\u3000\u2190\u3000\u672c\u4eba\u3082\u518d\u73fe\u3067\u304d\u306a\u3044\u306e\u3060\u304b\u3089\u306d
\n\u2463\u672c\u4eba\u304c\u8ad6\u6587\u3068\u304a\u308a\u306e\u65b9\u6cd5\u3067\u4f5c\u308c\u306a\u3044\u3000\u2190\u3000\u4e8b\u5b9f\u3060
\n\u2464\u5b66\u3068\u307f\u5b50\u304c\u554f\u984c\u306b\u3059\u308b\u610f\u5473\u304c\u308f\u304b\u3089\u306a\u3044\u3000\u2190\u3000\u5984\u60f3\u3060\u304b\u3089
\n\u2465\u9178\u6f2c\u3051\u306e\u65b9\u6cd5\u3092\u660e\u78ba\u306b\u3057\u3066\u3001\u3069\u3046\u3057\u3066\u9055\u3044\u304c\u3042\u308b\u306e\u304b\u3001\u3069\u3046\u3057\u3066\u518d\u73fe\u3059\u308b\u306e\u306b\u30af\u30ea\u30c6\u30a3\u30ab\u30eb\u306a\u306e\u304b\u306e\u8aac\u660e\u3092\u3057\u3066\u9802\u6234\u3000\u2190\u3000\u8981\u6c42\u3060
\n\u2466\u5b66\u3068\u307f\u5b50\u306eSTAP\u7d30\u80de\u306f\u8ad6\u6587\u306e\u5b9a\u7fa9\u306eSTAP\u7d30\u80de\u3068\u306f\u7570\u306a\u308b\u304b\u3089\u3001\u5b66\u3068\u307f\u5b50\u306eSTAP\u7d30\u80de\u306e\u5b9a\u7fa9\u3082\u66f8\u3051\u3000\u2190\u3000\u8981\u6c42\u3060
\n\u3067\u3059\u304c\u3001\u3069\u308c\u304c\u4e00\u822c\u4eba\u304c\u99ac\u9e7f\u306b\u3059\u308b\u767a\u8a00\u306a\u3093\u3067\u3057\u3087\u306d\uff1f<\/p>\n","protected":false},"excerpt":{"rendered":"

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