{"id":24764,"date":"2023-11-07T13:27:20","date_gmt":"2023-11-07T04:27:20","guid":{"rendered":"https:\/\/nbsigh2.com\/?p=24764"},"modified":"2023-11-24T10:19:37","modified_gmt":"2023-11-24T01:19:37","slug":"pcr%e3%81%a8%e9%9b%bb%e6%b0%97%e6%b3%b3%e5%8b%95","status":"publish","type":"post","link":"https:\/\/nbsigh2.com\/?p=24764","title":{"rendered":"PCR\u3068\u96fb\u6c17\u6cf3\u52d5"},"content":{"rendered":"

\u30ad\u30e1\u30e9\u5b50\u306bAcr\/CAG-GFP \u3068\u3044\u3046\u633f\u5165\u907a\u4f1d\u5b50\u3068\u67d3\u8272\u4f53\u306e\u6b20\u5931\u306e\u6709\u7121\u3092\u8abf\u3079\u3001\u30ad\u30e1\u30e9\u5b50\u306fES\u7d30\u80de\u3001\u4eca\u56de\u306fFES1\u7531\u6765\u3067\u3042\u308b\u3068\u3044\u3046\u8a3c\u660e\u3092\u3059\u308b\u305f\u3081\u3001\u6842\u8abf\u67fb\u59d4\u54e1\u4f1a\u306fPCR\u3068\u305d\u306e\u7523\u7269\u3092\u96fb\u6c17\u6cf3\u52d5\u3067\u8abf\u3079\u305f\u308f\u3051\u3067\u3059\u3002<\/p>\n

\u305d\u306e\u8abf\u3079\u65b9\u306b\u3064\u3044\u3066\u5b66\u3068\u307f\u5b50\u306f\u307b\u3068\u3093\u3069\u7406\u89e3\u3067\u304d\u3066\u304a\u3089\u305a\u3001\u7121\u99c4\u53e3\u4e0e\u592a\u90ce\u3082\u9593\u9055\u3048\u3066\u3044\u308b\u3053\u3068\u3092\u3001\u5f53\u65b9\u3084plus99%\u3055\u3093\u304c\u6307\u6458\u3057\u3066\u304d\u305f\u3068\u3053\u308d\u3067\u3059\u3002<\/p>\n

\u5f53\u65b9\u306e\u89e3\u8aac\u3092\u8aad\u3093\u3067\u5b66\u3068\u307f\u5b50\u306f\u65b0\u3057\u3044\u8a18\u4e8b<\/a>\u3092\u7acb\u3061\u4e0a\u3052\u307e\u3057\u305f\u3002\u5f53\u65b9\u306e\u30d6\u30ed\u30b0\u3082\u8a18\u4e8b\u306e\u30b3\u30e1\u30f3\u30c8\u304c100\u4ef6\u306b\u8fd1\u3065\u3044\u305f\u306e\u3067\u3001\u3053\u306e\u65b0\u3057\u3044\u8a18\u4e8b\u3092\u7acb\u3061\u4e0a\u3052\u308b\u3053\u3068\u306b\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u3044\u3064\u3082\u306e\u3088\u3046\u306b\u5b66\u3068\u307f\u5b50\u306f\u5f53\u65b9\u3084plus99%\u3055\u3093\u306e\u89e3\u8aac\u3092\u8aad\u3093\u3067\u3001\u305d\u3093\u306a\u3053\u3068\u306f\u524d\u304b\u3089\u77e5\u3063\u3066\u3044\u308b\u3068\u3070\u304b\u308a\u306b\u66f8\u304f\u308f\u3051\u3067\u3059\u3002\u5618\u3064\u304d\u3067\u3059\u304b\u3089\u3001\u5f53\u65b9\u7b49\u304b\u3089\u60c5\u5831\u3092\u5f97\u305f\u3068\u306f\u7d76\u5bfe\u3044\u308f\u306a\u3044\u306e\u3067\u3059\u3002\u3057\u304b\u3057\u307e\u3060\u7406\u89e3\u3067\u304d\u3066\u306a\u3044\u304b\u3089\u9593\u9055\u3044\u304c\u3042\u308b\u308f\u3051\u3067\u3059\u306d\u3002<\/p>\n

\u300c\u305f\u3081\u606f\u30d6\u30ed\u30b0\u306f\u3001\u5b66\u3068\u307f\u5b50\u306e\u9593\u9055\u3044\u3055\u304c\u3057\u306b\u8840\u773c\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u9055\u3044\u307e\u3059\u306a\u3002\u4f55\u56de\u3082\u8a00\u3044\u307e\u3059\u304c\u304b\u3063\u3071\u3048\u3073\u305b\u3093\u306a\u3093\u3067\u3059\u306a\u3002\u5b66\u3068\u307f\u5b50\u306f\u5f53\u65b9\u7b49\u304c\u5225\u306b\u304a\u9858\u3044\u3059\u308b\u3053\u3068\u3082\u306a\u304f\u3001\u304b\u3063\u3071\u3048\u3073\u305b\u3093\u3092\u4f9b\u7d66\u3057\u3066\u304f\u308c\u3066\u3044\u308b\u306e\u3067\u3059\u3002<\/p>\n

\u300cPCR\u53cd\u5fdc\u306b\u3088\u308b\u5897\u5e45\u306f\u3069\u306e\u3088\u3046\u306a\u4ed5\u7d44\u307f\u304b\uff1f\u306b\u3064\u3044\u3066\u3001\u305f\u3081\u606f\u30d6\u30ed\u30b0\u306f\u3001\u305d\u306e\u4ed5\u7d44\u307f\u3092\u77e5\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u300d<\/span>\u3000\u2190\u3000\u3061\u304c\u3044\u307e\u3059\u306d\u3002\u77e5\u3089\u306a\u304b\u3063\u305f\u306e\u306f\u5b66\u3068\u307f\u5b50\u306e\u65b9\u3067\u3059\u3002\u5f53\u65b9\u7b49\u306e\u8aac\u660e\u3092\u8aad\u3093\u3067\u7406\u89e3\u3067\u304d\u3066\u304d\u305f\u306e\u3067\u3059\u3002\u305d\u306e\u8a3c\u62e0\u306b\u4eca\u56de\u306e\u5b66\u3068\u307f\u5b50\u306e\u8a18\u4e8b\u306e\uff11\uff11\u6708\uff17\u65e5\uff08\u706b\uff09\u5348\u5f8c\uff11\uff13\u6642\u73fe\u5728\u306e\u8a18\u4e8b\u306e\u6700\u5f8c\u306f\u5f53\u65b9\u306e\u30b3\u30e1\u30f3\u30c8\u306e\u8ee2\u8f09\u3067\u3001\u3053\u306e\u5f53\u65b9\u306e\u30b3\u30e1\u30f3\u30c8\u306b\u5bfe\u3057\u3066\u306e\u53cd\u8ad6\u306f\u4e00\u3064\u3082\u3042\u308a\u307e\u305b\u3093\u3002\u305b\u3044\u305c\u3044\u300c\u7e70\u308a\u8fd4\u3059\u3053\u3068\u306b\u3088\u308aforward primer \u3068\u3000reverse primerm\u306e\u9593\u306eDNA\u9396\u304c\u6307\u6570\u95a2\u6570\u7684\u306b\u5897\u3048\u308b\u308f\u3051\u3067\u3059\u3002\u300d\u3068\u66f8\u3044\u3066\u3044\u307e\u3059\u304c\u3001\u3053\u3093\u306a\u3053\u3068\u306f\u8ab0\u3067\u3082\u3059\u3050\u306b\u7406\u89e3\u3067\u304d\u307e\u3059\u304b\u3089\u3001\u7121\u99c4\u306a\u8aac\u660e\u3067\u3059\u3002\u7279\u306b\u3001\u4e0b\u7dda\u90e8\u5206\u306e100bp\u306b\u3053\u3060\u308f\u308b\u8a18\u8f09\u306f\u4e0d\u8981\u3067\u3059\u3002\u300d<\/span>\u3000\u304f\u3089\u3044\u3057\u304b\u66f8\u3051\u306a\u3044\u306e\u3067\u3059\u3002<\/p>\n

\u300cplus\u3055\u3093\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u3067\u306f\u3055\u3080\u3068\u3044\u3046\u4ed5\u7d44\u307f\u304c\u7406\u89e3\u3067\u304d\u305a\u300120\u5869\u57fa\u3042\u308c\u3070\u3001\u30d0\u30e9\u30d0\u30e9\u306b\u306a\u3063\u3066\u3044\u308b\u907a\u4f1d\u5b50\u69cb\u9020\u306e\u7279\u5b9a\u90e8\u4f4d\u3092\u9078\u3076\u3053\u3068\u304c\u3067\u304d\u308b\u306e\u3060\u304b\u3089\u3001\u305d\u308c\u3060\u3051\u3067\u5341\u5206\u306a\u3093\u3060\u3068\u3044\u3046\u6975\u8ad6\u306b\u306a\u3063\u3066\u3057\u307e\u3057\u305f\u3002\u300d<\/span>\u3000\u2190\u5b66\u3068\u307f\u5b50\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u304c\u7406\u89e3\u3067\u304d\u3066\u3044\u306a\u3044\u304b\u3089plus99%\u3055\u3093\u306e\u5869\u57fa\u304c\uff12\uff10\u30b1\u4f4d\u306e\u5869\u57fa\u914d\u5217\u304c\u3042\u308c\u3070\u3001\u5168DNA\u9396\u306e\u4e2d\u304b\u3089\u7279\u5b9a\u306e\u90e8\u4f4d\u3092\u7279\u5b9a\u3067\u304d\u308b\u3068\u3044\u3046\u3053\u3068\u304c\u308f\u304b\u3089\u306a\u3044\u3088\u3046\u3067\u3059\u3002<\/p>\n

\u300c\u7aef\u306b\u8d64\u3042\u308b\u3044\u306f\u9752\u90e8\u5206\u304c\u4ed8\u3044\u305fDNA\u914d\u5217\u306e\u69cb\u9020\u4f53\u3068\u306a\u308c\u3070\u3001\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306f\u3001\u8d64\u3001\u3042\u308b\u3044\u306f\u9752\u69cb\u9020\u3092\u611f\u77e5\u3057\u3066\u3001\u305d\u3053\u3067\u4f38\u9577\u53cd\u5fdc\u3092\u3084\u3081\u307e\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u7406\u89e3\u3067\u304d\u3066\u306a\u3044\u8a3c\u62e0\u3067\u3059\u3002\u8d64\u3001\u9752\u306e\u69cb\u9020\u4f53\u3068\u306f\u306a\u3093\u3060\u304b\u308f\u304b\u308a\u307e\u305b\u3093\u304c\u3001\u305d\u3093\u306a\u306e\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u92f3\u578b\u3068\u306a\u308bDNA\u9396\u306f\u305d\u306e\u524d\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u306e\u5408\u6210\u958b\u59cb\u3092\u793a\u3059\u30d7\u30e9\u30a4\u30de\u30fc\u306a\u306e\u3067\u3001\u3053\u308c\u4ee5\u4e0a\u92f3\u578b\u306b\u306a\u308b\u90e8\u5206\u304c\u306a\u3044\u306e\u3067\u5408\u6210\u304c\u6b62\u307e\u308b\u306e\u3067\u3059\u3002\u591a\u5206\u3001\u3053\u306e\u8aac\u660e\u3067\u306f\u5b66\u3068\u307f\u5b50\u306b\u306f\u308f\u304b\u3089\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3089\u3001\u3082\u3063\u3068\u52c9\u5f37\u3057\u3066\u7406\u89e3\u3067\u304d\u305f\u3089\u3001\u3053\u306e\u5f53\u65b9\u306e\u8868\u73fe\u306e\u610f\u5473\u304c\u7406\u89e3\u3067\u304d\u308b\u3067\u3057\u3087\u3046\u3002
\n\u8ffd\u8a18\uff1a\u305f\u3081\u606f\u306f\u89aa\u5207\u3060\u304b\u3089\u5c0a\u5927\u306a\u304a\u30de\u30cc\u30b1\u306b\u3082\u308f\u304b\u308b\u3088\u3046\u306b\u8aac\u660e\u77e5\u3066\u3042\u3052\u307e\u3057\u305f\uff08\u4e0b\u8a18\uff09\u3002<\/p>\n

\u300c\u3053\u3053\u306e\u7406\u89e3\u304c\u3084\u3084\u96e3\u3057\u3044\u306e\u3067\u3001\u305f\u3081\u606f\u30d6\u30ed\u30b0\u306f\u7406\u89e3\u3067\u304d\u306a\u304b\u3063\u305f\u306e\u3067\u3059\u3002
\n\u4ed6\u306b\u3082\u3044\u308d\u3044\u308dPCR\u53cd\u5fdc\u8aac\u660e\u56f3\u306f\u3042\u308a\u307e\u3059\u304c\u3001\u3053\u306e\u56f3\u304c\u3001\u300c\u306a\u305c\u3001\u631f\u3081\u305f\u90e8\u5206\u306e\u307f\u304c\u5897\u5e45\u3059\u308b\u306e\u304b\uff1f\u300d\u306e\u7406\u5c48\u3092\u826f\u304f\u8aac\u660e\u3067\u304d\u3066\u3044\u307e\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u304a\u7b11\u3044\u3067\u3059\u306a\u3002\u3069\u3093\u306a\u8aac\u660e\u306b\u3082\u8d64\u9752\u306e\u69cb\u9020\u4f53\u304c\u3042\u308b\u304b\u3089\u5408\u6210\u304c\u505c\u6b62\u3059\u308b\u306a\u3069\u3068\u3044\u3046\u8aac\u660e\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n

\u300c\u3057\u304b\u3057\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u69cb\u9020\u3082\u542b\u307f\u306f\u3055\u307e\u308c\u305f\u90e8\u5206\u304c\u3001\u306a\u305c\u9078\u629e\u7684\u306b\u5897\u5e45\u3057\u3066\u3044\u304f\u304b\u3092\u3057\u3089\u306a\u3044\u4eba\u306f\u591a\u304b\u3063\u305f\u3067\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u5b66\u3068\u307f\u5b50\u3060\u3051\u304c\u77e5\u3089\u306a\u304b\u3063\u305f\u306e\u3067\u3059\u3002\u7686\u3055\u3093\u77e5\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u300c\u7aef\u306b\u30d7\u30e9\u30a4\u30de\u30fc\u90e8\u5206\u304c\u3042\u308c\u3070\u3001\u4f38\u9577\u53cd\u5fdc\u306f\u3001\u305d\u306e\u69cb\u9020\u3092\u611f\u77e5\u3057\u3066\u6b62\u307e\u308b\u3068\u3044\u3046\u7406\u5c48\u3092\u3001\u5f7c\u3089\u306f\u77e5\u3089\u306a\u304b\u3063\u305f\u306e\u3067\u3059\u3002\u300d<\/span>\u3000\u2190\u3000\u5b8c\u5168\u306b\u5a2f\u89e3\u3057\u3066\u307e\u3059\u306d\u3002\u305d\u308c\u4ee5\u4e0a\u92f3\u578b\u306b\u306a\u308b\u90e8\u5206\u304c\u306a\u3044\u304b\u3089\u6b62\u307e\u308b\u306e\u3067\u3059\u3002<\/p>\n

\u300c\u9577\u3044DNA\u914d\u5217\u3068\u3001\u77ed\u3044DNA\u914d\u5217\u304c\u306a\u305c\u3001\u540c\u3058\u3088\u3046\u306b\u898b\u3048\u308b\u306e\u304b\u300d<\/span>\u3000\u2190\u3000\u5b66\u3068\u307f\u5b50\u306f\u307e\u3060\u6b20\u5931\u90e8\u305d\u306e\u3082\u306e\u304c\u5897\u5e45\u3055\u308c\u308b\u304b\u3089\u9577\u3044\u914d\u5217\u304c\u3042\u308b\u3068\u601d\u3063\u3066\u3044\u308b\u3088\u3046\u3067\u3059\u3002\u7e70\u308a\u8fd4\u3057\u8aac\u660e\u3057\u305f\u306e\u306b\u308f\u304b\u3089\u306a\u3044\u3088\u3046\u3067\u3059\u306a\u3002<\/p>\n

\u4e00\u65b9\u3001\u3053\u3063\u3061\u306e\u30d0\u30ab\u304c\u559a\u3044\u3066\u3044\u307e\u3059\u3002
\n\u7121\u99c4\u53e3\u4e0e\u592a\u90ce\u66f0\u304f\uff1a\u30a2\u30eb\u30c4\u30cf\u30a4\u30de\u30fc\u91ce\u90ce\u304c\u3001\u3060\u3063\u305f\u3089\u3069\u3046\u3057\u3066TCR\u518d\u69cb\u6210\u30d0\u30f3\u30c9\u306f\u30e9\u30c0\u30fc\u306b\u306a\u308b\u306e\u3060\u3002\u9577\u3055\u304c\u9055\u3046\u304b\u3089\u3060\u308d\u3046\u304c\u3088\u3002\u304a\u524d\u306f\u3082\u3046\u5f15\u9000\u305b\u3044\u3002\u793e\u4f1a\u306e\u5bb3\u60aa\u3060\u3002 <\/a>
\nPCR\u7d50\u679c\u3092\u96fb\u6c17\u6cf3\u52d5\u3067\u793a\u3059\u3068\u3044\u3046\u7406\u5c48\u304c\u5168\u304f\u7406\u89e3\u3067\u304d\u3066\u306a\u3044\u306e\u3067\u3059\u306a\u3002\u8907\u6570\u306e\u9577\u3055\u306e\u7570\u306a\u308bDNA\u9396\u304c\u3042\u308b\u3068\u304d\u3001\u3053\u308c\u3092\u96fb\u6c17\u6cf3\u52d5\u3059\u308b\u3068\u3001\u5869\u57fa\u306e\u9577\u3055\u306b\u3057\u305f\u304c\u3063\u3066DNA\u9396\u306f\u79fb\u52d5\u3059\u308b\u306e\u3067\u3059\u3002\u305d\u306e\u7d50\u679c\u96fb\u6c17\u6cf3\u52d5\u306e\u56f3\u306b\u306f\u8907\u6570\u306e\u30d0\u30f3\u30c9\u304c\u51fa\u73fe\u3057\u3001\u68af\u5b50\uff1d\u30e9\u30c0\u30fc\u3068\u3044\u3046\u308f\u3051\u3067\u3059\u306a\u3002PCR\u306e\u7d50\u679c\u306f\u3001\u7279\u5b9a\u306e\u9577\u3055\u306eDNA\u9396\u304c\u3067\u304d\u308b\u308f\u3051\u3067\u3059\u304b\u3089\u3001\u30d0\u30f3\u30c9\u306f\u4e00\u3064\u3060\u3051\u3067\u3059\u3002\u3042\u308b\u3044\u306fPCR\u3067\u5897\u5e45\u3067\u304d\u308b\u92f3\u578b\u304c\u306a\u304b\u3063\u305f\u306e\u3067\u30d0\u30f3\u30c9\u306f\u306a\u3057\u3067\u3059\u3002All or Nothing \u306a\u306e\u3067\u3059\u3002\u3053\u308c\u304c\u308f\u304b\u3089\u306a\u3044\u306e\u3067\u3059\u304b\u306d\u3002\u7406\u89e3\u3067\u304d\u3066\u3044\u306a\u3044\u306e\u306b\u7f75\u5012\u3059\u308b\u304b\u3089\u3001\u30d0\u30ab\u3068\u3057\u304b\u8a55\u4fa1\u3055\u308c\u306a\u3044\u306e\u3067\u3059\u306a\u3002<\/p>\n

############################
\nPCR\u3067\u4e00\u5b9a\u306e\u9577\u3055\u306eDNA\u9396\u304c\u3067\u304d\u308b\u308f\u3051\u3002<\/h6>\n

\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u62e1\u5927\u3055\u308c\u307e\u3059\u3002
\n\"\"<\/a>
\n\u9ed2\u3044\u7dda\u304c\u30aa\u30ea\u30b8\u30ca\u30eb\u306eDNA\u9396\u3067\u3059\u3002F-P\u306fforward primer\u3001R-P\u306f\u3000reverse primer \u3067\u3059\u3002DNA\u9396\u306b\u306f\u65b9\u5411\u6027\u304c\u3042\u3063\u3066\uff13\u2019\u3001\uff15\u2019\u3068\u8868\u8a18\u3057\u307e\u3059\u304c\u7169\u308f\u3057\u3044\u306e\u3067\u7701\u3044\u3066\u3044\u307e\u3059\u3002\u8ffd\u8a18\uff1a\u5b66\u3068\u307f\u5b50\u304c\u7406\u89e3\u3067\u304d\u3066\u306a\u3044\u306e\u3067\u6539\u8a02\u3057\u307e\u3057\u305f\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u92f3\u578bDNA\u306b\u7d50\u5408\u3059\u308b\u306e\u3068\u3001\u5408\u6210\u3055\u308c\u305fDNA\u9396\u306e\u4e00\u90e8\u306b\u306a\u3063\u3066\u3044\u308b\u9055\u3044\u3082\u793a\u3057\u3066\u3044\u307e\u3059\u3002
\n\u52a0\u71b1\u3067\u4e56\u96e2\u3057\u305fDNA\u9396\u306b\u8a2d\u8a08\u3057\u305fforward primer\u3068reverse primer\u304c\u4ed8\u304d\u307e\u3059\uff08A)\u3002DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u305d\u308c\u305d\u308c\u77e2\u5370\u306e\u65b9\u5411\u3078DNA\u9396\u3092\u5408\u6210\u3057\u307e\u3059\uff08B)\u3002\u5408\u6210\u304c\u7d42\u4e86\u3059\u308b\u306e\u306f\u6e29\u5ea6\u3092\u4e0b\u3052\u3066DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u5931\u6d3b\u3059\u308b\u307e\u3067\u3067\u3059\u3002\u30aa\u30ec\u30f3\u30b8\u306eDNA\u9396\u304c\u3067\u304d\u305f\u308f\u3051\u3067\u3059\u3002\u3053\u3053\u3067\u52a0\u71b1\u3059\u308b\u3068forward primer\u3068reverse primer\u304c\u518d\u3073\u7d50\u5408\u3057\u307e\u3059\u3002\u7d50\u5408\u3059\u308b\u90e8\u4f4d\u306f\u30aa\u30ea\u30b8\u30ca\u30eb\u306eDNA\u9396\u3067\u306f\u524d\u3068\u540c\u3058\u3067\u3001\u30aa\u30ec\u30f3\u30b8\u306eDNA\u9396\u306b\u306f\u56f3\u306e\u3088\u3046\u306a\u90e8\u4f4d\u306b\u7d50\u5408\u3057\u307e\u3059\uff08C)\u3002DNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u304c\u50cd\u304f\u3088\u3046\u306b\u6e29\u5ea6\u3092\u4e0a\u3052\u308b\u3068\u3001\u30aa\u30ec\u30f3\u30b8\u306eDNA\u9396\u306f\uff11\u56de\u76ee\u3068\u540c\u69d8\u306b\u5408\u6210\u3055\u308c\u3066\u3044\u304d\u307e\u3059\u304c\u3001\u3053\u308c\u306b\u52a0\u3048\u3066\u30aa\u30ec\u30f3\u30b8\u306eDNA\u9396\u304c\u92f3\u578b\u306b\u306a\u308a\u30d4\u30f3\u30af\u77e2\u5370\u306e\u3088\u3046\u306a\u65b9\u5411\u306b\u5408\u6210\u304c\u59cb\u307e\u308a\u307e\u3059\u3002\u3053\u306e\u30d4\u30f3\u30af\u306eDNA\u9396\u306e\u5408\u6210\u306f\u30aa\u30ec\u30f3\u30b8\u306eDNA\u9396\u304c\u5408\u6210\u3092\u958b\u59cb\u3057\u305f\u3068\u304d\u306b\u3067\u304d\u305f\u90e8\u5206\uff08\u30d7\u30e9\u30a4\u30de\u30fc\uff09\u307e\u3067\u9032\u307f\u307e\u3059\u304c\u3001\u305d\u308c\u304b\u3089\u5148\u306f\u92f3\u578b\u306b\u306a\u308bDNA\u9396\u304c\u306a\u3044\u306e\u3067\u3053\u3053\u3067\u505c\u6b62\u3057\u307e\u3059\uff08\uff12\u30b5\u30a4\u30af\u30eb\u76ee\u306e\u7d42\u4e86\uff1aD\uff09\u3002\u7d50\u679c\u30d4\u30f3\u30af\u306e\u9577\u3055\u306eDNA\u9396\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u5408\u6210\u304c\u505c\u6b62\u3059\u308b\u306e\u306f\u306a\u306b\u304b\u505c\u6b62\u3055\u305b\u308b\u69cb\u9020\u4f53\u304c\u3042\u308b\u304b\u3089\u3067\u306f\u306a\u3044\u306e\u3067\u3059\u3002\u92f3\u578b\u304c\u306a\u3044\u304b\u3089\u3067\u3059\u3002\uff13\u30b5\u30a4\u30af\u30eb\u4ee5\u964d\u3067\u306f\u3001\uff12\u30b5\u30a4\u30af\u30eb\u76ee\u540c\u69d8\u30aa\u30ec\u30f3\u30b8\u3092\u92f3\u578b\u3068\u3059\u308b\u306e\u306b\u52a0\u3048\u3001\u30d4\u30f3\u30af\u306eDNA\u9396\u3092\u92f3\u578b\u306b\u3057\u3066\u30d4\u30f3\u30af\u306eDNA\u9396\u304c\u3064\u304f\u3089\u308c\u308b\u308f\u3051\u3067\u3059\u3002\u3057\u305f\u304c\u3063\u3066\uff4e\u30b5\u30a4\u30af\u30eb\u3092\u7e70\u308a\u8fd4\u3059\u3068\u3001\u30aa\u30ea\u30b8\u30ca\u30eb\u306b\u306e\u9ed2\u3044DNA\u9396\u306f\uff12\u672c\u306e\u307e\u307e\u304b\u308f\u3089\u305a\u3001\u30aa\u30ec\u30f3\u30b8\u306eDNA\u9396(2n-2)\u672c\u3067\u304d\u3001\u30d4\u30f3\u30af\u306eDNA\u9396\u306f(2^n-2n)\u672c\u3067\u304d\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u30d4\u30f3\u30af\u306eDNA\u9396\u304c\u6307\u6570\u95a2\u6570\u7684\u306b\u5897\u52a0\u3057\u3066\u3044\u304d\u307e\u3059\u3002<\/p>\n

\u306f\u3044\u3001\u5b66\u3068\u307f\u5b50\u69d8\u304a\u308f\u304b\u308a\u3067\u3057\u3087\u3046\u304b\uff1f\u305d\u3093\u306a\u306e\u306f\u308f\u304b\u3063\u3066\u3044\u308b\u306a\u3069\u3068\u6c7a\u3057\u3066\u8a00\u308f\u306a\u3044\u3067\u304f\u3060\u3055\u3044\u3002\u300c\u8d64\u3001\u3042\u308b\u3044\u306f\u9752\u69cb\u9020\u3092\u611f\u77e5<\/strong>\u3057\u3066\u3001\u305d\u3053\u3067\u4f38\u9577\u53cd\u5fdc\u3092\u3084\u3081\u307e\u3059\u3002\u300d<\/span>\u3068\u8a00\u3063\u305f\u306e\u3067\u3059\u304b\u3089\u306d\u3002\u7406\u89e3\u3067\u304d\u305f\u306e\u306a\u3089\u3001\u304a\u304b\u3052\u3055\u307e\u3067\u7406\u89e3\u3067\u304d\u305f\u3068\u8a00\u3046\u306e\u3067\u3059\u3088\u3002\u793e\u4f1a\u4eba\u306e\u5e38\u8b58\u3067\u3059\u304b\u3089\u306d\u3002<\/p>\n

\u52d5\u753b<\/a>\u306e\uff11\u5206\u301c\u3000\u3092\u898b\u3066\u307f\u306a\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u30ad\u30e1\u30e9\u5b50\u306bAcr\/CAG-GFP \u3068\u3044\u3046\u633f\u5165\u907a\u4f1d\u5b50\u3068\u67d3\u8272\u4f53\u306e\u6b20\u5931\u306e\u6709\u7121\u3092\u8abf\u3079\u3001\u30ad\u30e1\u30e9\u5b50\u306fES\u7d30\u80de\u3001\u4eca\u56de\u306fFES1\u7531\u6765\u3067\u3042\u308b\u3068\u3044\u3046\u8a3c\u660e\u3092\u3059\u308b\u305f\u3081\u3001\u6842\u8abf\u67fb\u59d4\u54e1\u4f1a\u306fPCR\u3068\u305d\u306e\u7523\u7269\u3092\u96fb\u6c17\u6cf3\u52d5\u3067\u8abf\u3079\u305f\u308f\u3051\u3067\u3059\u3002 \u305d\u306e\u8abf\u3079\u65b9\u306b\u3064\u3044 … \u7d9a\u304d\u3092\u8aad\u3080 PCR\u3068\u96fb\u6c17\u6cf3\u52d5<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2,8],"tags":[],"class_list":["post-24764","post","type-post","status-publish","format-standard","hentry","category-education","category-fake"],"_links":{"self":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/24764","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24764"}],"version-history":[{"count":24,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/24764\/revisions"}],"predecessor-version":[{"id":24791,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/24764\/revisions\/24791"}],"wp:attachment":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24764"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24764"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24764"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}