{"id":23846,"date":"2023-02-24T08:20:51","date_gmt":"2023-02-23T23:20:51","guid":{"rendered":"https:\/\/nbsigh2.com\/?p=23846"},"modified":"2023-03-02T14:56:29","modified_gmt":"2023-03-02T05:56:29","slug":"%e6%96%87%e7%ab%a0%e3%81%8c%e3%83%94%e3%83%aa%e3%82%aa%e3%83%89%e3%81%a7%e5%8c%ba%e5%88%87%e3%82%89%e3%82%8c%e3%81%9f%e3%82%89%e3%80%81%e6%af%8e%e5%9b%9e%e3%80%81%e3%81%9d%e3%81%ae%e6%9d%a1%e4%bb%b6","status":"publish","type":"post","link":"https:\/\/nbsigh2.com\/?p=23846","title":{"rendered":"\u6587\u7ae0\u304c\u30d4\u30ea\u30aa\u30c9\u3067\u533a\u5207\u3089\u308c\u305f\u3089\u3001\u6bce\u56de\u3001\u305d\u306e\u6761\u4ef6\u3092\u5165\u308c\u3088\uff01"},"content":{"rendered":"

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\n\u3053\u308c\u3092\u8aad\u3093\u3060\u5b66\u3068\u307f\u5b50\u306f\uff1b
\n<\/p>\n

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<\/a>\u3068\u3044\u3046\u308f\u3051\u3067\u3059\u3002<\/p>\n

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\n\u3055\u305b\u305f\u671f\u9593\u304c\u3042\u308b\u3068\u306f\u8ab0\u3082\u8aad\u307e\u306a\u3044\u3067\u3057\u3087\u3046\u306d\u3002\u3082\u3057\u3042\u308b\u306e\u306a\u3089\u3001\u3044\u3064\u3001\u8ab0\u304c\u6df7\u5165\u3055\u305b\u305f\u306e\u304b\u3092\u554f\u984c\u306b\u3057\u3066\u3044\u308b\u30bb\u30af\u30b7\u30e7\u30f3\u306a\u306e\u3067\u8a18\u8f09\u3059\u308b\u306b\u6c7a\u307e\u3063\u3066\u3044\u308b\u3067\u3057\u3087\u3046\u304c\u3002\u7279\u306b\u8a18\u8f09\u304c\u306a\u3044\u3068\u3044\u3046\u3053\u3068\u306f\u3053\u306e\uff17\u65e5\u9593\u3060\u3068\u3044\u3063\u3066\u3044\u308b\u3068\u7406\u89e3\u3059\u308b\u306e\u304c\u5f53\u7136\u3067\u3059\u3002<\/p>\n

Nature Article \u306e\u3000METHODS \u306e Chimaeric mouse generation and analyses.\u306e\u30ad\u30e1\u30e9\u4f5c\u6210\u306e\u624b\u9806\u306e\u6587\u7ae0\u306f<\/p>\n

When the STAP conversion conditions (low pH) were applied to CD451 lymphocytes, most day-7 clusters that were large and contained more than a few dozen small cells were positive for Oct4 (although the expression level varied). Therefore, we used only well-formed characteristic clusters (large ones) for this type of study and cut them by microknife to prepare donor cell clusters in a proper size for glass needle injection.<\/p><\/blockquote>\n

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