{"id":11504,"date":"2018-04-08T19:44:47","date_gmt":"2018-04-08T10:44:47","guid":{"rendered":"http:\/\/seigi.accsnet.ne.jp\/sigh\/blog\/?p=11504"},"modified":"2021-12-26T13:00:52","modified_gmt":"2021-12-26T04:00:52","slug":"diff-%e3%82%92%e4%bd%bf%e3%81%a3%e3%81%a6%e3%81%bf%e3%82%88%e3%81%86","status":"publish","type":"post","link":"https:\/\/nbsigh2.com\/?p=11504","title":{"rendered":"diff \u3092\u4f7f\u3063\u3066\u307f\u3088\u3046"},"content":{"rendered":"

\u8ad6\u6587\u3068\u8a00\u308f\u305a\u6587\u7ae0\u306e\u527d\u7a83\u3092\u30c1\u30a7\u30c3\u30af\u3059\u308b\u4e00\u3064\u306e\u65b9\u6cd5\u3068\u3057\u3066\u3001\u3068\u3082\u304b\u304f\u3001\uff12\u3064\u306e\u6587\u66f8\u306e\u6587\u5b57\u5217\u306e\u5dee\u7570\u3092\u6bd4\u8f03\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u306e\u30b5\u30a4\u30c8\u306b\u300c\u30c6\u30ad\u30b9\u30c8\u6bd4\u8f03\u30c4\u30fc\u30eb difff\u300a\uff83\uff9e\uff6d\uff8c\uff8c\u300b<\/a>\u300d\u3068\u3044\u3046\u4fbf\u5229\u306a\u30b5\u30a4\u30c8\u304c\u3042\u308a\u307e\u3059\u3002\u5b66\u751f\u30ec\u30dd\u30fc\u30c8\u306e\u527d\u7a83\u30c1\u30a7\u30c3\u30af\u306b\u4fbf\u5229\u3067\u3059\u3002\u4f7f\u3044\u65b9\u306f\u7c21\u5358\u3067\u3001\u5de6\u53f3\u306e\u30da\u30a4\u30f3\u306b\u6bd4\u8f03\u3059\u308b\u305d\u308c\u305e\u308c\u306e\u6587\u5b57\u5217\u3092\u30b3\u30d4\u30fc\u30da\u30fc\u30b9\u30c8\u3057\u3001\u300c\u6bd4\u8f03\u3059\u308b\u300d\u30dc\u30bf\u30f3\u3092\u30af\u30ea\u30c3\u30af\u3059\u308c\u3070\u3044\u3044\u306e\u3067\u3059\u3002\u7570\u306a\u308b\u90e8\u5206\u304c\u685c\u8272\uff08\u5b63\u7bc0\u9650\u5b9a\uff09\u3067\u8868\u793a\u3055\u308c\u307e\u3059\u3002\u3061\u3087\u3046\u3069\u3044\u3044\u30b5\u30f3\u30d7\u30eb\u304c\u3067\u304d\u305f\u306e\u3067\u4f7f\u3063\u3066\u307f\u307e\u3057\u3087\u3046\u3002
\n\u5f53\u65b9\u304c\u52dd\u624b\u306b\u8a71\u984c\u306b\u3057\u3066\u3044\u308b\u5973\u533b\u306e\u8a18\u4e8b<\/a>\u306e2018.4.7\u73fe\u5728\u7248\u306f\uff1b<\/p>\n

I read the book titled \u201cthat day\u201d in Japanese written by Mis Obokata.
\nThe book contains Obokata’s memories during working as a researcher in Riken Wakayama laboratory in Kobe.
\nSTAP story had many mysterious events, finally she was accused to be the most suspicious person who contaminated ES cell. Offical committee did not comfirm her guilty of ES contamination, but committee’s conclusion harmed her seriously.
\nAs the result she suffered from depression. Therefore she decided to publish her book describing about her experience in Riken after 2 years of the miscondut event.
\nShe wrote her book as memories style,\u3000but, actually her book asked Dr Wakayama to reveal his manipulation about STAP experiments.
\nThe most important points in her book is that she did not participate making STAP stem cells.
\nAt first, she asked Dr Wakayama to reveal what kinds of mouse strain she was given prior to STAP cell preparing.
\nShe wrote in her book that she used the mice just she was given from Dr Wakayama without knowing its strain, therefore, she did not check its strain at all.
\nIn the past press conference at June 16 in 2014, Dr Wakayama said he himself or other staffs in the laboratory prepared the mouse. For preparing STAP cells, she just used the mouse given by Dr Wakayama or other staffs in the laboratory.
\nInitially, Dr Wakayama passed her the mouse directly, later, other staff did so.
\nDr Wakayama said he did not leave any mouse preparing records. Katsura official investigation committee confirmed Obokata did not know any mice strain at all.
\nFurther, she was confused why the officially analyzed results of STAP (stem) cell gene differed from those of mouse she used in the experiment. She was not able to mention the reason of these gaps. She wrote Dr Wakayama was the only parson who could tell the reason because he made STAP stem cell alone.
\nIn the course of STAP experiments, Dr Wakayama succeeded STAP stem cells conversion from STAP cells. He succeeded STAP stem cell experiments in a row.
\nTherefore, Obokata’s part of STAP cell experiments was exclusively restricted.
\nShe just made STAP cell through lethal processing with acid stress by using spleen lymphocytes taken from young mice.
\nDr Wakayama made STAP stem cell named FLS. Then he made Fgf induce stem cells named CTS or FI which were able to make fetus and placenta.
\nShe wrote that STAP stem cell experiments were done by Dr Wakayama himself without Obokata\u2019s Cooperation.
\nShe was not thought how to use manipulator at all in Wakayama laboratory, therefore she did not make STAP stem cells.
\nAfter all, she was not allowed to participate STAP stem cell and related experiments.
\nShe tried to increase STAP cell by adapting STAP cell to artificial medium but failed.
\nFor her, it was almost impossible to culture STAP cells in the artificial ES or TS medium.
\nActually, she continued to struggle with STAP cells culturing without success.
\nAccording to the contract, Wakayama laboratory moved to the new laboratory in Yamanasi University in March 2013.
\nAfter Wakayama laboratory leaving, Dr Sasai helped Obokata to finish the rewrote version of STAP manuscript to Nature.
\nThen, Mis Obokata sent the finished version of the manuscript to Nature in March 2013.
\nNature requested her to send the STAP manuscript after correction of several pointed contents.
\nShe struggled to make revised version of STAP manuscript according to Dr Sasai and Dr Niwa\u2019s advises.
\nUnfortunately, she was not able to contact Dr Wakayama who actually conducted STAP stem cell experiments.
\nFinally, Nature accepted STAP manuscript in December 2013.<\/p><\/blockquote>\n

\u3067\u3057\u305f\u3002
\n\uff12\u65e5\u5f8c\u306e2018.4.9\u73fe\u5728\u3067\u306f\u65ad\u308a\u306a\u3057\u306b\u4ee5\u4e0b\u306e\u3088\u3046\u306b\u6539\u8a02\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n

I read the book titled \u201cthat day\u201d in Japanese written by Mis Obokata, so I would like to introduce this book, here.
\nI apologize the story written below is just my understanding or impression after reading.
\nThe book contains Obokata’s memories during working as a researcher in Riken Wakayama laboratory in Kobe.
\nSTAP story had many mysterious events, finally she was accused to be the most suspicious person who contaminated ES cell\u3000in STAP cells.
\nOffical committee did not confirm ES contamination was her conduct, but this committee’s ambiguous conclusion harmed her seriously.
\nAs the result, she suffered from serious depression, so called post-traumatic stress disorder (PTSD). Therefore she decided to write several events she experienced in Riken laboratory.
\nTwo years later, the book titled “That day” was published.
\nShe wrote her book as a memory story, actually, she asked Dr Wakayama to tell facts that were regarded as unresolved.
\nShe would also like to know the possibilities of his manipulation about STAP experiments.
\nThe most important points in her book is that she did not participate making STAP stem cells.
\nTherefore, she asked Dr Wakayama to reveal the mouse strain she was given prior to STAP cell preparing at first.
\nShe wrote in her book that she used the mice just she was given from Dr Wakayama.
\nIn the past press conference at June 16 in 2014,
\nDr Wakayama said he himself or other staffs in the laboratory prepared the mouse.
\nFor preparing STAP cells, she used the mouse given by just Dr Wakayama or other staffs in the laboratory.
\nInitially, Dr Wakayama passed her the mouse directly, later, other staff did so.
\nShe did not know the experimental mice strain, therefore, she did not check its strain at all. It was almost impossible for her to tell the reason of mouse gene gap when she was asked after STAP experiment.
\nDr Wakayama said he did not leave any records of the mouse prepared.
\nKatsura official investigation committee confirmed Obokata did not know any mice strain at all.
\nFurther, she was confused why the officially analyzed genes of STAP (stem) cells differed from those of experimental mouse she used.
\nShe was not able to mention the reason of these gene gaps.
\nDr Wakayama was the only person who could tell the gene gap between STAP (stem) cell and used animal because he made STAP stem cell alone.
\nIn the course of STAP experiments, Obokata was informed that Dr Wakayama succeeded making kimera mouse and STAP stem cells establishment.
\nHe did the serial STAP stem cell experiments in with his colleagues.
\nConsidering these facts, Obokata’s experimental part was exclusively restricted to preparing STAP cells.
\nShe just made STAP cell through lethal processing with acid stress by using spleen lymphocytes taken from newborn mice.
\nDr Wakayama made STAP stem cell named FLS. Then he made Fgf induce stem cells named CTS or FI which were able to make fetus and placenta.
\nShe was not taught how to use manipulator at all in Wakayama laboratory, therefore she did not make STAP stem cells technically.
\nAfter all, she was not allowed to participate STAP stem cell and related experiments.
\nShe insisted in her book that STAP stem cell experiments were done by Dr Wakayama himself.
\nOn the other hand, Obokata tried to increase STAP cells by the method of adapting the cells to artificial medium.
\nShe was not able to grow STAP cell in the artificial ES or TS medium.
\nIt was almost impossible for her to culture STAP cells for the purpose of conversion from STAP cell to STAP stem cell.
\nActually, she continued to struggle with STAP cells culturing without success.
\nAccording to the contract, Wakayama laboratory moved to the new laboratory in Yamanasi University in March 2013.
\nAfter Wakayama laboratory leaving, Dr Sasai helped Obokata to write the new version of STAP manuscript sending to Nature.
\nThen, Mis Obokata sent the two new versions of STAP manuscripts to Nature in March 2013.
\nNature requested her to send back STAP revised manuscript after correction of several pointed contents.
\nShe struggled to make revised version of STAP manuscript. She did additional experiments according to Dr Sasai and Dr Niwa\u2019s help.
\nUnfortunately, she was not able to contact Dr Wakayama who actually conducted STAP stem cell experiments.
\nKatsura official investigation committee did not reveal these process of STAP stem cell experiments.
\nFinally, Nature accepted STAP manuscript in December 2013.<\/p><\/blockquote>\n

\u306a\u3093\u305b<\/p>\n

2018\/4\/6(\u91d1) \u5348\u5f8c 11:58 \u5b66\u3068\u307f\u5b50
\n\u79d8\u5bc6\u88e1\u306b\u76f4\u3057\u307e\u3059<\/p><\/blockquote>\n

\u3068\uff08oTake\u3055\u3093\u66f0\u304f\uff09\u3088\u304f\u308f\u304b\u3089\u306a\u3044\u5ba3\u8a00<\/a>\u3092\u3057\u3066\u3044\u307e\u3059\u3057\u3001\u500b\u4eba\u306e\u30d6\u30ed\u30b0\u306a\u3093\u3067\u7a81\u7136\u5909\u66f4\u3055\u308c\u3066\u3082\u6587\u53e5\u306a\u3093\u304b\u3044\u3044\u307e\u305b\u3093\u3088\u3002
\n\u9d8f\u3082\u3082\u3092\u30d5\u30e9\u30a4\u30d1\u30f3\u3067\u76ae\u76ee\u3092\u713c\u304d\u3001\u713c\u304d\u8272\u304c\u3064\u3044\u305f\u3089\u3072\u3063\u304f\u308a\u8fd4\u3057\u3066\u65e5\u672c\u9152\u3092\u6ce8\u3044\u3067\uff17\u5206\u3001\u84cb\u3092\u3057\u3066\u5f31\u706b\u3067\u84b8\u3057\u713c\u304d\u306b\u3057\u307e\u3059\u3002\u307f\u3058\u3093\u5207\u308a\u7345\u5b50\u5510\u3092\u30b5\u30e9\u30c0\u6cb9\u3067\u7092\u3081\u3001\u9577\u30cd\u30ae\u307f\u3058\u3093\u5207\u308a\uff0b\u8c46\u677f\u91a4\uff0b\u65e5\u672c\u9152\uff0b\u7802\u7cd6\uff0b\u9162\uff0b\u91a4\u6cb9\u3092\u52a0\u3048\u3066\u3055\u3089\u306b\u7092\u3081\u305f\u30bd\u30fc\u30b9\u3092\u304b\u3051\u3066\u3044\u305f\u3060\u3044\u3066\u3044\u308b\u6700\u4e2d\u3067\u3059\u3002\u3053\u306e\u30bd\u30fc\u30b9\u3001\u30aa\u30ea\u30b8\u30ca\u30eb\u306f\u571f\u4e95\u5584\u6674\u3055\u3093\u306e\u3068\u3093\u304b\u3064\u306b\u304b\u3051\u308b\u30bd\u30fc\u30b9<\/a>\u306a\u3093\u3067\u3059\u3051\u3069\u9d8f\u8089\u3067\u3082\u7f8e\u5473\u3057\u3044\u3067\u3059\u3002\u30c8\u30f3\u30ab\u30c4\u3067\u306f\u306a\u304f\u5869\u80e1\u6912\u3057\u305f\u3060\u3051\u306e\u9d8f\u3082\u3082\u8089\u3092\u84b8\u3057\u713c\u304d\u3057\u305f\u3082\u306e\u306b\u304b\u3051\u308b\u65b9\u304c\u3044\u3044\u3068\u601d\u3044\u307e\u3057\u305f\u3088\u3002\u30c8\u30f3\u30ab\u30c4\u3067\u8a66\u3057\u3066\u3044\u307e\u305b\u3093\u3051\u3069\u306d\u3002\u5199\u771f\u64ae\u308a\u305d\u3053\u306d\u305f\u3002
\n\u9591\u8a71\u4f11\u984c
\nplus99% \u3055\u3093\u306e\u30b3\u30e1\u30f3\u30c8\u3092\u53d6\u308a\u5165\u308c\u305f\u3093\u3067\u3057\u3087\u3046\u304b\u306d\u3001\u304b\u306a\u308a\u6539\u5909\u3055\u308c\u3066\u3044\u307e\u3059\u3002
\n\u3061\u3087\u3046\u3069\u3044\u3044\u30b5\u30f3\u30d7\u30eb\u306a\u3093\u3067\u3001\u8208\u5473\u3042\u308b\u65b9\u306f diff \u3092\u4f7f\u3063\u3066\u307f\u305f\u3089\u3069\u3046\u3067\u3057\u3087\u3002
\n\"\"<\/a>
\n\u3053\u3093\u306a\u7d50\u679c\u306b\u306a\u308b\u3067\u3057\u3087\u3046\u3002
\nplus99% \u3055\u3093\u306e\u610f\u898b\u306b\u5f93\u3044\u3001\u3053\u306e\u8a18\u4e8b\u3092\u30a2\u30c3\u30d7\u3057\u305f\u7406\u7531\u3092\u6700\u521d\u306b\u52a0\u7b46\u3057\u305f\u3088\u3046\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u305d\u308c\u306b\u7d9a\u3044\u3066\u300cI apologize the story written below is just my understanding or impression after reading. \u300d\u3060\u3063\u3066\u3002\u3060\u308c\u3082\u307e\u3060\u975e\u96e3\u3057\u3066\u3044\u306a\u3044\u306e\u306b\u3001\u4f55\u6545\u6700\u521d\u304b\u3089\u8b1d\u308b\u3093\u3067\u3057\u3087\u3046\u306d\u3002\u81ea\u5206\u306e\u4e3b\u5f35\u306f\u975e\u96e3\u3055\u308c\u308b\u3082\u306e\u3060\u3068\u3044\u3046\u8a8d\u8b58\u304c\u3042\u308b\u3093\u3067\u3059\u304b\u306d\u3002\u4e0d\u601d\u8b70\u306a\u65b9\u3067\u3059\u3002
\n\u5f53\u65b9\u304c\u6307\u6458\u3057\u305f\u30b9\u30da\u30ea\u30f3\u30b0\u30df\u30b9\u306f\u307e\u3060\u76f4\u3063\u3066\u3044\u307e\u305b\u3093\u306d\u3002\u300cdepression\u306b\u306a\u3063\u305f\u304b\u3089\u8d77\u8349\u3057\u305f\u300d\u304c\u300cdepression\u306b\u306a\u3063\u3066PTSD\u306b\u306a\u3063\u305f\u304b\u3089\u8d77\u8349\u3057\u305f\u300d\u306b\u5909\u308f\u3063\u3066\u3044\u307e\u3059\u304c\u3001\u5927\u3057\u305f\u5909\u5316\u3067\u306f\u306a\u3044\u3067\u3059\u3002\u305d\u3093\u306a\u3053\u3068\u306f\u672c\u4eba\u306f\u3082\u3068\u3088\u308a\u8ab0\u3082\uff08\u5b66\u3092\u9664\u304f\uff09\u8a00\u3063\u3066\u3044\u307e\u305b\u3093\u3002
\n\u5148\u306b\u3082\u8ff0\u3079\u307e\u3057\u305f\u304c\u3001\u610f\u5473\u4e0d\u660e\u306b\u304d\u307e\u3063\u3066\u3044\u308b\u306e\u3067\u3001\u6700\u5f8c\u307e\u3067\u8aad\u3080\u6c17\u306f\u306a\u3044\u308f\u3051\u3067\u3059\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\t\t\t\t\t\t<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2,8],"tags":[],"class_list":["post-11504","post","type-post","status-publish","format-standard","hentry","category-education","category-fake"],"_links":{"self":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/11504","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11504"}],"version-history":[{"count":1,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/11504\/revisions"}],"predecessor-version":[{"id":21798,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/11504\/revisions\/21798"}],"wp:attachment":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11504"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=11504"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=11504"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}