{"id":1102,"date":"2011-02-25T21:13:50","date_gmt":"2011-02-25T12:13:50","guid":{"rendered":"http:\/\/mike.md.tsukuba.ac.jp\/terui\/blog\/?p=1102"},"modified":"2021-12-28T14:34:04","modified_gmt":"2021-12-28T05:34:04","slug":"%e9%9b%bb%e6%b0%97%e6%b3%b3%e5%8b%95%e7%94%bb%e5%83%8f%e3%81%ae%e6%8d%8f%e9%80%a0","status":"publish","type":"post","link":"https:\/\/nbsigh2.com\/?p=1102","title":{"rendered":"Western blotting\u753b\u50cf\u306e\u634f\u9020"},"content":{"rendered":"<p>Western blotting\u306e\u7d50\u679c\u306f\u3001\u8ad6\u6587\u306b\u63b2\u8f09\u3059\u308b\u305f\u3081\u306b\u306f\u4e0d\u5fc5\u8981\u306a\u90e8\u5206\u306f\u30ab\u30c3\u30c8\u3059\u308b\u3002\u539f\u56f3\u306f\u6614\u306f\u9280\u5199\u771f\u3067\u3042\u3063\u305f\u304c\u3001\u6700\u8fd1\u306f\u30b9\u30ad\u30e3\u30ca\u3067\u53d6\u308a\u8fbc\u3080\u306e\u304c\u3042\u305f\u308a\u307e\u3048\u3001\u3064\u307e\u308a\u30c7\u30b8\u30bf\u30eb\u60c5\u5831\u306a\u308f\u3051\u3060\u3002\u3060\u304b\u3089Photoshop\u3067\u52a0\u5de5\u3059\u308b\u3053\u3068\u306b\u306a\u308b\u3002<br \/>\n\u3067\u3001\u9b54\u304c\u5dee\u3059\u3068\u90fd\u5408\u306e\u3044\u3044\u3088\u3046\u306b\u6539\u3056\u3093\u51fa\u6765\u3061\u3083\u3046\u306e\u3060\u3002\u5370\u5237\u7269\u3068\u304bpdf\u306b\u306a\u308b\u3068\u3001\u3082\u3046\u5207\u308a\u8cbc\u308a\u306e\u8de1\u306f\u3088\u307b\u3069\u3057\u3064\u3053\u304f\u898b\u306a\u3044\u3068\u308f\u304b\u3089\u306a\u3044\u3002peer reviewer\u00a0\u3060\u3063\u3066\u634f\u9020\u3055\u308c\u3066\u3044\u306a\u3044\u304b\u3069\u3046\u304b\u306f\u30c1\u30a7\u30c3\u30af\u3057\u306a\u3044\u3057\u3001\u3067\u304d\u306a\u3044\u3002peer reviewer \u306f\u8ad6\u6587\u306e\u8ad6\u7406\u3068\u304b\u306b\u6ce8\u610f\u304c\u884c\u304f\u306e\u3067\u3001\u3088\u3082\u3084\u753b\u50cf\u304c\u634f\u9020\u3055\u308c\u3066\u3044\u308b\u3068\u306f\u601d\u308f\u306a\u3044\u3002<br \/>\n\u3053\u308c\u306f\u3001\u634f\u9020\u304c\u554f\u984c\u3068\u306a\u3063\u305f\u8ad6\u6587\u306epdf \u3092\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3057\u3066\u3042\u308b\u56f3\u306e\u4e00\u90e8\u306e\u753b\u50cf\u3092\u753b\u9762\u30ad\u30e3\u30d7\u30c1\u30e3\u3057\u3066\u8abf\u3079\u3066\u307f\u305f\u3082\u306e\u3060\u3002<br \/>\n<a href=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-capture.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-22243 size-medium\" src=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-capture-300x192.jpg\" alt=\"\" width=\"300\" height=\"192\" srcset=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-capture-300x192.jpg 300w, https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-capture-768x493.jpg 768w, https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-capture.jpg 859w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p style=\"text-align: left;\">\u3053\u306e\u56f3\u3092\u898b\u3066\u3001\u540c\u3058\u30d0\u30f3\u30c9\u304c\u3069\u3053\u304b\u306b\u3042\u308b\u306a\u3069\u306f\u3001\u3059\u3050\u306b\u306f\u5206\u304b\u3089\u306a\u3044\u3060\u308d\u3046\u3002\u3057\u304b\u3057\u3001\uff12\u968e\u8abf\u5316\u3057\u3066\u898b\u308b\u3068<\/p>\n<p><a href=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-2modes.gif\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-22244 size-medium\" src=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-2modes-300x193.gif\" alt=\"\" width=\"300\" height=\"193\" srcset=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-2modes-300x193.gif 300w, https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-2modes-768x493.gif 768w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p style=\"text-align: left;\">\u3069\u3046\u3060\u308d\uff1f\u8d64\u4e38\u306e\u4e2d\u306e\u56db\u89d2\u3044\u7a81\u8d77\u304c\u4f3c\u3066\u3044\u308b\u3068\u3059\u3050\u6c17\u304c\u3064\u304f\u3002\u3088\u304f\u307f\u308b\u3068\u3053\u306e\uff12\u3064\u306e\u30d0\u30f3\u30c9\u306f\u4ed6\u306e\u90e8\u5206\u3082\u307b\u3068\u3093\u3069\u540c\u3058\u3060\u3002<\/p>\n<p style=\"text-align: left;\">\u30c8\u30fc\u30f3\u30ab\u30fc\u30d6\u3067\u30c8\u30fc\u30f3\u3092\u5909\u5316\u3055\u305b\u3001\u3055\u3089\u306b\u8abf\u3079\u308b\u3068<\/p>\n<p><a href=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-tone-curve.gif\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-22245 size-medium\" src=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225-tone-curve-300x266.gif\" alt=\"\" width=\"300\" height=\"266\" \/><\/a><\/p>\n<p style=\"text-align: left;\">\u539f\u56f3\u306e\u4e0b\u306e\u5217\u306e\u5de6\u304b\u3089\uff12\u756a\u76ee\u3001\u306f\u305d\u306e\u5de6\u53f3\u306e\u30d0\u30f3\u30c9\u3068\u306e\u5883\u76ee\u304c\u4e0d\u81ea\u7136\u3060\u3002\u4ed6\u306e\u30d0\u30f3\u30c9\u306e\u5883\u76ee\u306b\u7e26\u306b\u8d70\u308b\u7dda\u304c\u306a\u3044\u306e\u306b\u3053\u3053\u3060\u3051\u306f\u3042\u308b\u3002\u4e00\u756a\u4e0a\u306e\u5217\u306e\u5de6\u304b\u3089\uff12\u3064\u76ee\u3092\u5207\u308a\u53d6\u308b\u3068\u304d\u3001\u305d\u306e\u5de6\u306e\u30d0\u30f3\u30c9\u306e\u4e00\u90e8\u3082\u542b\u3093\u3067\u3057\u307e\u3063\u305f\u3088\u3046\u3060\u3002<\/p>\n<p style=\"text-align: left;\">\u96fb\u6c17\u6cf3\u52d5\u306e\u30d0\u30f3\u30c9\u306a\u3093\u304b\u3001\u4f3c\u901a\u3063\u305f\u3082\u306e\u304c\u3067\u304d\u308b\u306e\u3060\u304b\u3089\u3001\u3053\u3046\u3044\u3046\u3053\u3068\u3092\u3084\u3063\u3061\u3083\u3046\u3093\u3060\u308d\u3046\u306a\u3002<\/p>\n<p style=\"text-align: left;\">\u3053\u3063\u3061\u306f\u3001\u307e\u305f\u5225\u306e\u4eba\u7269\u306e\u634f\u9020<\/p>\n<p><a href=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225original.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-22246 size-medium\" src=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225original-300x42.jpg\" alt=\"\" width=\"300\" height=\"42\" srcset=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225original-300x42.jpg 300w, https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225original-1024x142.jpg 1024w, https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225original-768x107.jpg 768w, https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/20110225original.jpg 1246w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p style=\"text-align: left;\">\u53f3\u534a\u5206\u3092\u5207\u308a\u53d6\u308a\u4e0a\u4e0b\u53cd\u8ee2\u3057\u3001\u3061\u3087\u3063\u3068\u5de6\u53f3\u3092\u5f15\u304d\u5ef6\u3070\u3057\u4e26\u3079\u3066\u307f\u308b\u3068\u540c\u4e00\u30c7\u30fc\u30bf\u3068\u3044\u3046\u306e\u304c\u308f\u304b\u308b\u3002<\/p>\n<p><a href=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/2011022check1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-22247 size-medium\" src=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/2011022check1-300x220.jpg\" alt=\"\" width=\"300\" height=\"220\" srcset=\"https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/2011022check1-300x220.jpg 300w, https:\/\/nbsigh2.com\/wp-content\/uploads\/2011\/02\/2011022check1.jpg 736w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p style=\"text-align: left;\">Western blotting\u00a0\u306e\u30c7\u30fc\u30bf\u306a\u3093\u304b\u3001\u4f7f\u3048\u306a\u3044\u3082\u306e\u3092\u542b\u3081\u3066\u3044\u3063\u3071\u3044\u3042\u308b\u3060\u308d\u3046\u304c\u3001\u306a\u306b\u3082\u540c\u4e00\u306e\u3082\u306e\u304b\u3089\u5207\u308a\u8cbc\u308a\u3059\u308b\u3053\u3068\u306f\u306a\u3044\u3060\u308d\u3046\u306b\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\t\t\t\t<![CDATA[]]>\t\t<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-1102","post","type-post","status-publish","format-standard","hentry","category-3"],"_links":{"self":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/1102","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1102"}],"version-history":[{"count":3,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/1102\/revisions"}],"predecessor-version":[{"id":22248,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=\/wp\/v2\/posts\/1102\/revisions\/22248"}],"wp:attachment":[{"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1102"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1102"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nbsigh2.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1102"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}